五种鲤科鱼类生长激素cDNA的克隆和序列分析  

Molecular Cloning and Sequence Analysis of the cDNAs Encoding Growth Hormone of Five Kinds of Fishes of Carp Family

作　　者：于辉[1] 李华[1] 颜其贵[2] 林涛[2] 冯婷[2] 冯迎春[2] 

机构地区：[1]佛山科技学院生命科学学院,广东佛山528231 [2]四川农业大学动物医学院,四川雅安625014

出　　处：《广东海洋大学学报》2008年第4期1-5,共5页Journal of Guangdong Ocean University

基　　金：广东省自然科学基金(7010423)

摘　　要：通过RT-PCR方法，以草鱼、鳙鱼、鲫鱼、鲤鱼、齐口裂腹鱼等5种鲤科重要经济鱼类的垂体总RNA为模板扩增出其生长激素（fish growth hormone，fGH）的完整ORF序列，并克隆到pMD18-T载体上，命名为pMD-1（草鱼）、pMD-2（鳙鱼）、pMD-3（鲫鱼）、pMD-4（鲤鱼）、pMD-5（齐口裂腹鱼）。测序结果显示，ORF序列其长度均为633bp。序列分析表明，所有ORF序列均以ATG为起始密码，以TAG为终止密码，编码210个氨基酸残基，推导的生长激素前体由22个氨基酸的信号肽和188个氨基酸的成熟肽组成。同源性分析表明，草鱼、鳙鱼、鲤鱼、鲫鱼和齐口裂腹鱼的fGH同源性在93．3％-99．5％之间。The 5 cDNAs encoding fish growth hormone （fGH） genes were amplified by RT-PCR from five important economic fishes, Ctenopharyngodonidella, Hypophthalmichthys molitrix, Carassius aureate, Cyprinus carpio and Schizothorax prenanti based on the total RNA obtained from pituitary gland, and were cloned to pMD18-T vector, and designated pMD-1, pMD-2, pMD-3, pMD-4 and pMD-5, respectively. It is the first time to clone GH cDNAs of S. prenant in China. The lengths of the open reading frame （ORF） of all the cDNAs are 633 bp. Sequence analysis showed that each complete ORF （eDNA） of the above fishes includes the same start codon of ATG and the same end codon of TAG, and encodes a protein of about 210 amino acids, which is composed of signal sequence of 22 amino acids and mature peptide sequence of 188 amino acids. Bioinformatics analysis showed that the identity offGH is located at 93.3%-99.5%.

关 键 词：鱼类生长激素(fGH) CDNA克隆 序列分析 草鱼 鳙鱼 鲫鱼 鲤鱼 齐口裂腹鱼 

分 类 号：Q75[生物学—分子生物学] Q959.468