星洲银罗非鱼线粒体细胞色素b基因的序列分析  

Sequence Analysis of Oreochromis sp. (Red Tilapia) Based on cyt b Gene in Mitochondrial DNA

作　　者：刘楚吾[1] 李路[1] 林晓坤 刘丽[1] 

机构地区：[1]广东海洋大学水产学院,广东湛江524025 [2]吴川市水产技术推广站,广东湛江524500

出　　处：《广东海洋大学学报》2008年第4期6-9,共4页Journal of Guangdong Ocean University

基　　金：湛江市农业重大科技项目(200415)

摘　　要：克隆并测定了星洲银罗非鱼（Oreochromis sp．）细胞色素b基因（cytb）的全序列，得到全长1141bp的序列用于分析，用MEGA4．0分析软件与GenBank中的线粒体DNA序列进行序列比较，显示星洲银罗非鱼与其他鱼类的cytb基因的同源性较高，同源度介于83％～93％间；根据星洲银罗非鱼与莫桑比克罗非鱼、尼罗罗非鱼等12种鱼类的cytb基因序列构建的系统进化树，初步确定尼罗罗非鱼、莫桑比克罗非鱼为星洲银罗非鱼的父母本。The complete sequence ofcyt b gene ofOreochromis sp. （ O. niloticus ×O. mossambicus ） was studied. 1 141bp sequence was obtained through PCR amplification, and was compared by MEGA 4.0 program with the mitochondrial DNA sequences downloaded from GenBank for comparison. The results showed that there was a close homology （83%--93%） for the cyt b gene sequence between Oreochromis sp. and other fishes. The phylogenetic tree was constructed based on cyt b gene of twelve fishes, such as Oreochromis sp., O. niloticus, 0. mossambicus etc. The parents （O. niloticus 9 , ♀. mossambicus ♂） and taxonomic position of Oreochromis sp. were initially affirmed through molecula data.

关 键 词：星洲银罗非鱼 线粒体 细胞色素B基因 基因序列 分子系统学 

分 类 号：Q75[生物学—分子生物学] Q959.483