3-甲基胆蒽对斑马鱼组织病理效应的初步研究  

Preliminary Studies on Histopathological Changes in Zebrafish Exposure to 3-Methylcholanthrene

作　　者：杨蓉[1] 聂芳红[2] 孔庆波[3] 谢英明[1] 林红英[1] 陈进军[1] 

机构地区：[1]广东海洋大学农业生物技术研究所,广东湛江524088 [2]广东海洋大学食品科技学院,广东湛江524088 [3]中国刑事警察学院警犬技术系,辽宁沈阳110034

出　　处：《广东海洋大学学报》2008年第4期91-94,共4页Journal of Guangdong Ocean University

基　　金：广东省自然科学基金项目"几种DLCs对斑马鱼CYP1A的生化毒理学机理研究"(05011789);广东省国际合作项目"斑马鱼生物学标志CYP1A在DLCs环境污染监测中的应用研究"(2007B050200023)

摘　　要：分别用25、50、100、200和400μg/L的3-甲基胆蒽(3-MC)对斑马鱼进行水浴染毒,于第7天和第14天采集各染毒组和对照组活鱼的肝、鳃、心、肠和肾,进行病理组织学检查。结果发现,一定剂量的3-MC能导致肝细胞变性,胞浆内出现脂肪滴,严重时肝细胞坏死解体,界限模糊;心肌细胞萎缩坏死;肠粘膜上皮细胞纹状缘不整齐,有的脱落;鳃丝结构异常,鳃小片表面粗糙,上皮细胞不完整;肾小管上皮细胞肿胀,细胞界限不清,管腔内均质红染。供试斑马鱼的病理组织变化与3-MC的作用剂量和作用时间存在一定的关系。A range of zebrafish （Danio rerio） were exposed to 3-methylcholanthrene （3-MC） by water pollution at the doses of 25 ug/L, 50 ug/L, 100ug/L, 200 ug/L and 400 ug/L, respectively. On the 7th day and 14tb day of exposure to 3-MC, the liver, gill, heart, intestine and kidney of the fish were collected to observe histopathological changes. It was found that the hepatocytes were denaturalized with fat drops in the cellular plasma and even showed necroses or disaggregation and obscure cellular borderlines, that there were atrophies and necroses in cardiac cells, that the villuses of the enteric epithelia were out of order and even fallen off, that the secondary lamella of the gill became coarse and some of the epithelia were lost, and that the epithelia of the renal tubules were swollen and the cellular borderlines were not clear, with even red dye in some tubules. It was also found that there were, to some extent, dose-effect andtime-effect relationships between the histopathological changes and 3-MC.

关 键 词：3-甲基胆蒽 毒性 病理组织学 斑马鱼 

分 类 号：R994.6[医药卫生—毒理学]