异丙酚靶控输注系统研究不同麻醉深度下兔脑c-fos mRNA水平的改变  

Alteration of C-fos mRNA Levels in Discrete Brain Regions of Rabbit under Different Anesthetic Depths by Using Target Controlled Infusion of Propofol

作　　者：陈建颜[1] 卢健芳[1] 高申山[1] 

机构地区：[1]广东省深圳市第二人民医院麻醉科,518035

出　　处：《中国误诊学杂志》2008年第27期6562-6564,共3页Chinese Journal of Misdiagnostics

摘　　要：目的:研究异丙酚不同麻醉深度对兔脑c-fos mRNA水平的影响。方法:30只日本大耳兔,随机分为对照组、浅麻醉组、深麻醉组,每组各10只。应用异丙酚靶控输注系统,研究异丙酚不同麻醉深度下大脑额叶皮质,小脑皮质浦肯野细胞层、颗粒细胞层c-fos mRNA表达水平的改变。结果:与对照组比较,大脑额叶皮质,小脑皮质浦肯野细胞层c-fos mRNA表达水平随着麻醉深度的增加而显著升高(P<0.05);小脑皮质颗粒细胞层c-fos mRNA表达水平在麻醉状态下显著升高(P<0.05),但麻醉深度的增加并不能进一步降低其灰度值。结论:异丙酚通过增加c-fos mRNA的表达,影响随后反应基因的转录而发挥抑制中枢的作用。异丙酚抑制中枢的作用部位存在区域性差异。Objective：To research the alterations of c-fos mRNA in discrete brain regions of rabbit during propofol anesthesia. Methods：30 rabbits were randomly divided into three groups with ten each ：controll group,light anesthetic group ,deep anesthetic group. By using target-controlled infusion of propofol,the levels of c-fos mRNA in frontal cortex （FC）,cerebellar purkinje cells layer （PCL）,cerebellar granule cortex layer （GCL） were observed under different anesthetic depths. Results ：With the increase of anesthetic depth,the levels of c-fos mRNA in FC and PCL increased significantly compared with controll group（P〈0.05）, The levels of c-fos mRNA in GCL increased significantly in both anesthetic groups（P^0.05） ,but the grayscale scores were not decreased further with the increase of anesthetic depth. Conclusions ：Propofol exerts its anesthetic action through increasing the expression of c-fos mRNA,subsequently working on fos-related gene transcription. This kind of influence differes in different brain regions.

关 键 词：二异丙酚/投药和剂量 麻醉药 静脉/投药和剂量 原癌基因蛋白质c-fos/遗传学/代谢 RNA 信使/代谢 兔 动物 

分 类 号：R614[医药卫生—麻醉学]