异丙酚对缺氧复氧后培养海马神经元nNOS的影响  

Effects of propofol on the anoxic and re-oxygen responses of primary cultured hippocampal neurons

作　　者：王彦[1] 杨静[1] 李天佐[1] 

机构地区：[1]首都医科大学附属北京同仁医院麻醉科,100730

出　　处：《北京医学》2008年第9期546-548,共3页Beijing Medical Journal

摘　　要：目的观察异丙酚对培养海马神经元缺氧复氧后损伤反应的影响。方法培养12d海马神经元,随机分为正常对照再培养24h组(Nor组)、缺氧4h后复氧24h组(Ano组)、加异丙酚500μmol/L缺氧4h后复氧24h组(Pro组)。采用MTT法测定细胞存活率,免疫组化方法测定nNOS蛋白含量,应用流式细胞仪测定神经元凋亡率。结果缺氧后Ano组nNOS蛋白表达及细胞凋亡率明显增加(P﹤0.01),加入异丙酚后能减少nNOS蛋白含量(P﹤0.01)和细胞凋亡率(P﹤0.05),Pro组细胞存活率较Ano组增加(P﹤0.05),但与Nor组比仍下降(P﹤0.05)。结论异丙酚能提高体外海马神经元的抗缺氧能力,减少nNOS蛋白过度表达和细胞的凋亡。Objective To evaluate the effects of propofol on the anoxic and re-oxygen responses of primary cultured hippocampal neurons. Methods Cultrued -12-day hippocampal cells were randomly divided into three groups： normal control group（Group Nor）, anoxia 4 hours and re-oxygen 24 hours group（Group Ano）, anoxia 4 hours and re-oxygen 24 hours with 500μmol/L propofol group （Group Pro）. Surviving-cell ratio was assayed by MTT, the expression of nNOS protein was measured by immunofleurescence staining and neuron apoptosis ratie was determined by flow cytometry for each group. Results The nNOS protein expression and apoptosis ratio in Group Pro were less than those in Group Ano. Surviving-ceU ratio in Group Pro was significantly higher than that in Group Ano （P 〈 0.01）, but lower than that in Group Nor. Conclusions Propofol may enhance the tolerance ability of primary cultured hippocampal neurons to anoxia by reducing the expression of nNOS protein and apoptosis of cells.

关 键 词：异丙酚 缺氧 海马神经元 一氧化氮合酶 

分 类 号：R96[医药卫生—药理学]