溃疡病菌对杨树愈伤组织细胞膜透性、可溶性糖及MDA含量的影响  

Effects of Infection with Botryosphaeria dothidea on Cell Membrane Permeability,Soluble Sugar and MDA Content in Poplar Calli

作　　者：梁军[1] 王媛[1] 贾秀贞[1] 张星耀[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林生态学与森林保护学重点实验室,北京100091

出　　处：《林业科学》2008年第8期72-77,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30671682);国家"十一五"林业科技支撑重点项目课题(2006BAD08A11);国家林业局重点项目(2005-10)

摘　　要：采用抗性水平不同的毛白杨和北京杨愈伤组织,用生理生化分析方法研究接种溃疡病菌后细胞膜透性、可溶性糖及MDA含量的动态变化。结果表明:溃疡病菌侵染后,2种杨树愈伤组织细胞膜透性上升,相对电导率显著增加,感病种上升幅度大于抗病种;可溶性糖含量的变化在抗/感病种间随时间变化差异较大,感病种可溶性糖含量呈上升趋势,在24h和120h达到高峰,抗病种可溶性糖含量48h时高于对照,但比相应对照增加幅度小,方差分析表明抗病种处理与对照间可溶性糖含量差异不显著;感病种MDA含量上升,而抗病种MDA含量变化不明显,说明感病种出现了明显的膜脂过氧化现象。Two poplar species with different resistance （ Populus tomentosa, a resistant one; P. beijingensis, a susceptible one） to a canker disease were used to study the dynamic changes of cell membrane permeability, soluble sugar and MDA content in the callus inoculated with the pathogen of Botryosphaeria dothidea. After inoculation, cell membrane permeability of two species increased with a significant raise of relative electrical conductivity, and the increment in the susceptible species was higher than that in the resistant one. The pattern of changes in soluble sugar with time varied in the two species. Soluble sugar content in susceptible species occurred two peaks, respectively at 24 h and 120 h. The resistant species had higher soluble sugar content than that of the uninfected control at 48 h, however its increment was smaller than the latter. There was no significant difference in the soluble sugar content between the inoculated and its control of the resistant species. MDA content increased in the susceptible species, while no significant change was observed in the resistant one. The result showed that serious lipid peroxidation occurred in the susceptible species.

关 键 词：毛白杨 北京杨 BOTRYOSPHAERIA dothidea 杨树愈伤组织 细胞膜透性 相对电导率 丙二醛 可溶性糖 

分 类 号：S718.43[农业科学—林学]