^(99)Tc^m-HL91乏氧显像预测高压氧放疗增敏的可行性  

Feasibility of ^(99)Tc^m-HL91 Hypoxia Imaging to Predict the Radiosensitization of Hyperbaric Oxygenation

作　　者：田艳[1] 曹艳霞[1] 

机构地区：[1]河北大学附属医院核医学科,河北保定071000

出　　处：《同位素》2008年第3期150-154,共5页Journal of Isotopes

摘　　要：为探讨99Tcm-HL91乏氧显像预测高压氧对肿瘤放疗增敏的可行性,将经过高压氧舱治疗后的荷H22肝癌KM小鼠按其出高压氧舱的时间分为15min组、30min组、60min组,以不进行高压氧治疗的小鼠为对照组。各组小鼠分别取5只进行99Tcm-HL91乏氧显像和60Co照射治疗,各组显像小鼠通过感兴趣区(ROI)技术,计算肿瘤与对侧相应部位放射性摄取比(T/NT);显像后立即处死小鼠,完整剥离肿瘤,称重并计算放射性计数,计算肿瘤微分摄取率(DUR)。各组照射治疗小鼠均记录肿瘤照射后生长天数及小鼠平均存活时间。结果显示,各组与对照组比较,高压氧舱治疗15min组、30min组的DUR、T/NT、肿瘤及小鼠生长延迟天数均有显著性差异(P<0.05);60min组各数值均未见显著性差异(P>0.05)。以上结果提示,99Tcm-HL91乏氧显像是一种有效预测高压氧增敏效果的方法,但高压氧增敏作用随出高压氧舱时间延长逐渐减弱,30min内增敏效果好,因此联合治疗时间越早越好。To observe the feasibility of 99 ^99Tc^m-HL91 hypoxia imaging to predict the radiosen- sitization of hyperbaric oxygenation, the mice bearing Hzz tumor KM were placed into hyperbaric oxygen chamber for a certain time, and were divided into three groups of 15 min, 30 min and 60 min, according to the time of being out of hyperbaric oxygenation. Five mice of every group were carried on ^99Tc^m-HL91 hypoxia imaging and ^60Co therapy respectively. The mice without hyperbaric oxygenation therapy were regarded as control group. The T/ NT of tumor to contralateral muscles were calculated by ROI technology for all of the imaging mice. After acquired, the mice were killed soon. The tumors were excised and weighted, and the radioactivity and differential uptake ratio（DUR） were computed by quantitating the tracer uptake. Every radiotherapy group mice were observed and recorded the tumors growth days and the average survival time. The results showed that the DUR T/ NT and the delay times of the tumors growth were significantly different in the 15 rain group and 30 min group respectively（P〈0.05）, compared with the control group. While there were no significant difference between the 60 min group and the control group（P〉 0.05）. The results showed that ^99Tc^m-HLgl hypoxia imaging is an effective method of predicting effects of hyperbaric oxygen enhancement. The sensitizing effect of hyperbaric oxygen is the best within the initial 30 rain then becomes decreasing gradually. The sooner combined treatments are adopted ,the better effects are.

关 键 词：^99TC^M-HL91 乏氧显像 高压氧 放射增敏 

分 类 号：R817[医药卫生—影像医学与核医学] R815[医药卫生—放射医学]