中草药有效成分的氚标记  

Study on Chinese Herbal Medicine Active Ingredients Labelled With Tritium

作　　者：董墨[1] 包广粮[1] 

机构地区：[1]中国科学院上海应用物理研究所,上海201800

出　　处：《同位素》2008年第3期161-164,共4页Journal of Isotopes

摘　　要：分别采用氚水交换与低负压气液交换2种标记方法,标记了中草药的3个有效成分:栀子苷元、乙酰阿卡宁和绿原酸;经TLC和HPLC分离分析,放化纯度均>95%,比活度分别为5.97、3.24和470GBq/g。以上结果表明,氚水交换法及低负压气液交换法成功标记了含活泼双键的中草药有效成分。ChineSe medicinal herb active ingredients was labeled with triteium by using exchange of new synthesized tritiated water or exchange of low-pressure gas-liquid. The active ingredients was Genipin, acetylalkannin and chlorogenic acid . The radiochemical purity of the three labeled compounds were more than 95% after TLC and HPLC purification. The specific activities of tritium labeled-genipin, acetylalkannin and chlorogenic acid were 5.97, 3.24 and 470 GBq/g, respectively. The results indicated that the unstable Chinese medicinal herb active ingredients could be labeled with tritium by the methods of exchange of new synthesized tritiated water and exchange of low-pressure gas-liquid.

关 键 词：氚标记 中草药有效成分 栀子苷元 乙酰阿卡宁 绿原酸 

分 类 号：R284.1[医药卫生—中药学] O615.4[医药卫生—中医学]