维生素A缺乏对肠粘膜细胞因子免疫应答的影响  

THE INFLUENCES OF VITAMIN A DEFICIENCY ON CYTOKINES RESPONSE OF INTESTINAL MUCOSA IN RATS

作　　者：袁亚杰[1] 杨毅[1] 王卫平[1] 

机构地区：[1]复旦大学附属儿科医院,上海200032

出　　处：《营养学报》2008年第4期369-373,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30371210);上海市科学技术委员会基金(No.06DZ22035)

摘　　要：目的研究维生素A缺乏(vitamin A deficiency,VAD)对大鼠肠粘膜细胞因子免疫应答的影响,探讨VAD降低粘膜免疫力的机制。方法在建立VAD大鼠模型的基础上,给予鼠伤寒沙门氏菌灌胃诱导肠道感染。取新鲜回肠组织,采用ELISA和RT-PCR法,从蛋白和基因水平测定IFN-γ、IL-2、IL-4、IL-6、IL-10和IL-12的变化。结果VAD大鼠肠粘膜主要由树突状细胞产生的细胞因子IL-12的表达显著增高,[蛋白(lg ng/g mucosal tissue)VA正常组4.07±0.15,VAD4.41±0.02,基因(lg copies/50ng total RNA)VA正常组4.99±0.18,VAD5.53±0.15,伴有肠道感染时IL-12的产生进一步升高,蛋白和基因两者分别为:4.59±0.15和5.78±0.26];Th1细胞因子:IFN-γ降低两者分别为蛋白:VA正常组3.15±0.34,VAD2.67±0.15;基因:VA正常组3.15±0.34,VAD2.67±0.15,和VA正常组5.36±0.26,VAD5.19±0.27,均(P<0.01);Th2细胞因子IL-4在肠道感染后显著降低(蛋白VAD组为2.29±0.13,VA正常组2.39±0.20),尤其是VAD大鼠IL-4水平更低(蛋白2.13±0.17);IL-6mRNA在肠道感染时都明显下降(感染后VA正常组5.45±0.3,感染后VAD组5.44±0.3,VA正常组5.85±0.31)(P<0.01),IL-10mRNA在未感染VAD大鼠也降低(VAD5.10±0.14,VA正常组5.78±0.26)(P<0.01),在肠道感染时降低更为显著(4.56±0.33)(P<0.01)。结论对肠粘膜局部细胞因子产生水平的调节可能是VAD影响粘膜免疫功能的重要机制。Objective To understand the mechanism of vitamin A deficiency （VAD） impairing the mucosal immunity and the influences of VAD on cytokines response of intestinal mucosa in rats. Method The rat model with VAD was established, and intestinal infection was induced by typhoid salmonella orally administered. The fresh ileum tissue samples were taken, and ELISA and RT- quantity PCR were used to detect the production of cytokines, including IFN-Y, IL-2, IL-4, IL-6, IL-10 and IL-12, by intestinal mucosa in protein and mRNA levels. Results IL-12, the cytokine produced by dendritic cells, was markedly increased in VAD rats, and the level was further raised when the VAD rats were complicated by intestinal infection. The Thl cytokines, IFN-y and IL-2, were reduced in VAD rats. IL-4, the main Th2 cytokine, was significantly decreased after intestinal infection, especially in VAD rats. IL-6 mRNA was lower in VAD rats and after intestinal infection compared with that of the VA normal control. IL-10 mRNA was also declined in VAD rats, and more markedly reduced after intestinal infection. Conclusion The regulation of VAD onproduction of cytokines in local intestinal mucosa is likely to be an important mechanism of VAD affecting intestinal mucosal immunity.

关 键 词：维生素A 肠道 粘膜免疫 感染 细胞因子 

分 类 号：R392[医药卫生—免疫学]