高效液相色谱测定构树叶中辅酶Q_(10)含量  

DETERMINATION OF COENZYME Q_(10) IN BROUSSONETIA PAPYRIFERA BY HIGH PERFORMANCE CHROMATOGRAPHY

作　　者：李万仓[1] 郑宏燕[2] 张惠娟[1] 张浩 曹玉广[1] 

机构地区：[1]华中科技大学同济公共卫生学院流行病与卫生统计系,武汉430030 [2]华中科技大学同济公共卫生学院,环境与健康研究平台武汉430030 [3]深圳市华士康科技有限公司,深圳518000

出　　处：《营养学报》2008年第4期417-419,共3页Acta Nutrimenta Sinica

摘　　要：目的建立一种测定构树叶辅酶Q10含量的方法。方法构树叶用75%乙醇提取,萃取,蒸馏,浓缩。色谱柱为C18250mm×4.6mm i.d10μm,流动相为无水乙醇-甲醇(65:35),流速为1ml/min,检测器波长275nm。结果辅酶Q10在4～64μg/ml(r=0.9997)范围内具有良好的线性关系。平均加样回收率为98.66%,RSD为0.97%(n=6)。构树叶中辅酶Q10的最低检测限为10ng/ml(S/N=3)。结论该法快速,简便,准确。适宜于构树叶辅酶Q10含量的检测。Objective A simple and rapid high performance liquid chromatographic （HPLC） method for the determination of coenzyme Ql0 （CoQ10） in Broussonetia paryifera was developed. Method The sample of Broussonetia paryifera leares was extracted by 75% ethanol, extraction, distillation, concentration. Hypersil C18 （250mmx 4.6 mm i.d 10 μm） was selected as the analytical column, ethanol-methanol （65：35 by volume） as the mobile phase with flow-rate of 1 ml/min and UV 275 nm as the detection wavelength. Results Thelinear range of the calibration curve of concentration vs. peak height was 4-64 μg/ml with a coefficient of determination was 0.9997. The average recovery rate was 98.66% and the RSD was 0.97% （n=6）. The detection limit of CoQ10 in Broussonetia paryifera was 10ng/ml （S/N=3）. Conclusion This method was rapid, simple and suitable for the determination of CoQ10 in Broussonetia paryifera.

关 键 词：构树叶 辅酶Q10(CoQ10) 高效液相色谱 

分 类 号：Q946[生物学—植物学]