SD大鼠腺性膀胱炎动物模型的建立  

The establishment of cystitis glandularis animal model in SD rats

作　　者：易憬[1] 熊飞[2] 苏良平[1] 李庭庭[2] 陈登[1] 章慧平[2] 杨兵[1] 张盛龙[1] 张宝珍[1] 刘长[1] 

机构地区：[1]武钢大冶铁矿医院,黄石435000 [2]华中科技大学同济医学院计划生育研究所

出　　处：《临床外科杂志》2008年第8期553-554,共2页Journal of Clinical Surgery

摘　　要：目的研究一种发病机制、病理改变与临床接近且周期较短的SD大鼠腺性膀胱炎模型制备方法与技术。方法模型组采用DH5α大肠埃希菌溶液（10^8～10^9CFU／100μL浓度），每隔2天灌注雌性SD大鼠膀胱以诱导产生腺性膀胱炎。对照组以生理盐水每隔2天灌注膀胱；空白组不采取任何干预措施，同条件饲养。45d后收集膀胱标本进行病理学检查。结果模型组膀胱标本的腺性膀胱炎发生率显著高于对照组及空白组（P〈0．001）；对照组与空白组其发生率差异没有统计学意义（P〉0．05）。结论使用大肠杆菌多次膀胱灌注雌性SD大鼠诱导产生的腺性膀胱炎动物模型其病理表现与人类腺性膀胱炎具有相似性，适用于临床腺性膀胱炎的研究。Objective To study the methods and techniques for establishment of cystitis glandularis animal model which is more time saving and similar to clinical process and mechanism of onset and pathological changes of cystitis glandularis. Methods Fifty female SD rats were randomized into three groups. E. coli solution was infused into the bladders of test rats within 2 days intermittently to induce cystitis glandularis, and normal saline into control group with the same method. No measurement was admitted to the blank group. The animals were bred under the same conditions. The bladders＇specimen was collected for pathological measurement in 45 days later. Results The incidence of cystitis glandularis was significantly higher in test group than that in other two groups （ P 〈 0. 001 ） ; but there was no significant difference between control group and bland group（ P 〉 0.05 ）. Conclusion E. coli infusion into female SD rats＇bladder can induce cystitis glandularis successfully with similar manifestation of pathological changes to human beings＇ and this technique can apply to clinical study of cystitis glandularis.

关 键 词：腺性膀胱炎 SD大鼠 动物模型 

分 类 号：R694.3[医药卫生—泌尿科学]