维生素B_2在硫酸溶液中的单扫描极谱氧化波及其应用  

Single Sweep Polarographic Oxidation Wave of Riboflavin in Sulfuric Acid and Its Application

作　　者：张芬[1] 朱琳[1] 罗登柏[1] 

机构地区：[1]中南民族大学化学与材料科学学院分析化学国家民委重点实验室,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2008年第2期1-3,共3页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(2005ABA001);中南民族大学自然科学基金重点资助项目(YZY06001)

摘　　要：报道了维生素B2在酸性溶液中的极谱氧化波及其应用．在0．12mol／L的硫酸溶液中，维生素B2在-0．04V（vs．SCE）处产生一个灵敏的吸附性氧化峰，其二阶导数波峰尖锐、对称，波高与0．025×10^-6～9．0×10^-6g／mL范围内的维生素B2呈线性关系，相关系数为0．997，检出限达0．020×10^-6g／mL对于5．0×10^-6g／mL的维生素B2平行进行11次测试，RSD为1．10％．极谱波的波高在室温下可以稳定8h．讨论了测定中干扰物质中的影响及机理包括循环伏安图，温度系数，表面活性剂的影响和电毛细管曲线．结果降低了检测下限．Riboflavin produces a sensitive oxidation wave at -0. 04 V （vs. SCE） in sulfuric acid. The second derivative peak is sharp and symmetrical. The wave height shows a good linear relationship to the concentration of Vitamin Bz in the range of 0. 025 × 10^-6 -9.0× 10^-6 g/mL, the correlation coefficient is 0. 997, and the detection limit is 0. 020× 10^-6 g/mL. The relative standard deviation for 11 independent determination of 5.0× 10^-6 g/mL riboflavin is 1. 10 0%, The polarographic wave was stable in 8 h. The effects of interfering materials and the mechanism including cyclic voltammograms, temperature coefficient and electrocapillary curves were discussed. Compared with literatures data, the lower limit was reduced.

关 键 词：维生素B2 单扫描极谱法 氧化波 

分 类 号：O675.14[理学—化学]