全血基因组DNA 3种提取方法的比较  

Comparison of Three Methods for Extracting Human Genomic DNA from Whole Blood

作　　者：胡婧[1] 袁媛[2] 陈思礼[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]武汉大学第二临床医学院,武汉430071

出　　处：《中南民族大学学报（自然科学版）》2008年第2期24-26,共3页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：中南民族大学自然科学基金资助项目(YZZ04006);国家民委自然科学重点基金资助项目(MZZ04002)

摘　　要：目的:比较从全血中3种提取DNA方法所需要的时间、样本量、提取的DNA纯度、产量以及方法本身的优缺点.方法:采用蛋白酶K法、氯仿-异戊醇法、碘化钾法直接从全血中提取基因组DNA.结果表明:3种方法所提取的DNA质量均能达到分子生物学的实验要求,但碘化钾法提取的DNA纯度最高,且操作时间最短,该法简便、快速和DNA收获率高.Three methods, protease K, chloroform-isoamyol and potassium iodide methods, for extracting human genomic DNA from whole blood were compared on the time required, sample size, DNA purity, quantification of DNA and their advantages and disadvantages. The qualities of the DNA extracted from all the three methods could meet the requirements of the molecular biological experiments. Potassium iodide method harvested the best quality DNA and required the least time. Potassium iodide method was simple, rapid and efficient, and could be used for routine DNA extraction from whole human blood from the findings.

关 键 词：全血 基因组DNA 提取 不同方法的比较 

分 类 号：Q781[生物学—分子生物学]