丹参大黄合剂对拟老年痴呆大鼠学习记忆能力及海马β淀粉样前体蛋白、早老素1mRNA表达的影响  

Effects of Chinese herb Danshen-Dahuang on learning,memory ability and hippocampal gene expression of APP and PS1 in rats with Alzheimer disease

作　　者：韩柏[1] 唐荣华[1] 王锋[2] 赵书越[3] 韩德五[2] 

机构地区：[1]华中科技大学同济医学院同济医院神经科,武汉430030 [2]山西医科大学肝病研究所 [3]山西医科大学第一医院精神科

出　　处：《中华临床医师杂志（电子版）》2008年第8期35-38,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：山西省留学基金项目(2000-10-85);山西省科技攻关项目(2007031092-8);山西省留学基金项目(2007-13-89)

摘　　要：目的探讨丹参大黄合剂对拟老年性痴呆(AD)大鼠学习记忆能力的影响及其机制。方法用D-半乳糖和三氯化铝联合用药制备拟AD动物模型。从造模的第20天开始,丹参大黄组灌胃给予丹参大黄合剂,连续70d。给药结束后,通过方形水迷宫、逆转录聚合酶链反应来观察丹参大黄合剂对拟AD模型大鼠学习记忆和海马β淀粉样前体蛋白(APP)、早老素1(PS1)基因表达的影响。结果丹参大黄合剂可以缩短拟AD模型大鼠水迷宫测试的潜伏期(P<0.05),减少其错误次数(P<0.05),同时降低海马APP、PS1 mRNA的表达(P<0.05)。结论丹参大黄合剂能提高拟AD大鼠学习、记忆能力,可能与降低APP、PS1 mRNA的表达有关。Objective To investigate the effect of Chinese herb Danshen-Dahuang on learning and memory ability in rats with Alzheimer disease （AD） induced by D-galactose and AlCl3 and its possible mechanisms.Methods The AD model was produced by injecting D-galactose and AlCl3 intraperitoneally for 90 days.Since the 20th day of D-galactose and AlCl3 intraperitoneal injection,the rats in Danshen-Dahuang group had been treated with Danshen-Dahuang extraction by intragastric administration for 70 days.Subsequently,learning and memory ability of the mice was evaluated by Morris water maze and hippocampal gene expression of APP,PS1 was tested by reverse transcription polymerase chain reaction （RT-PCR）.Results Rats intragastric administration with Danshen-Dahuang,mice had shorter latency （P〈0.05） and less error times （P〈0.05） in water maze test compared with those in AD model group.At the same time,Danshen-Dahuang down regulated the expression of APP,PS1 mRNA （P〈0.05） in hippocampus.Conclusions Danshen-Dahuang improves the learning and memory ability of AD rats,its mechanism may be related to the downregulated expression of APP,PS1 mRNA.

关 键 词：丹参△ 大黄△ 大鼠 Wistar 阿尔茨海默病 学习 记忆 海马 淀粉样β-蛋白前体 早老素类 

分 类 号：R285[医药卫生—中药学]