镉致肾小管上皮细胞损伤时胞内肌动蛋白与ATP的改变及意义  

ALTERATION OF ATP AND F-ACTIN IN RENAL TUBULAR CELLS TREATED WITH CADMIUM

作　　者：姜傥[1,2] 谭敏[1,2] 关伟明 谭炳德[1,2] 董秀清 

机构地区：[1]中山医科大学附属第一医院 [2]广东省职业病防治院

出　　处：《职业卫生与应急救援》1997年第3期117-120,共4页Occupational Health and Emergency Rescue

基　　金：国家自然科学基金

摘　　要：从细胞功能与结构入手，着重观察镉染毒后细胞内能量代谢变化及其与细胞骨架、细胞形态学改变之间的关系。方法：采用离体肾灌注镉染毒模型，以免疫荧光及超微病理技术对肾小管细胞内肌动蛋白结构行时效分析。结果：染毒早期，肌动蛋白结构可迅速改变，且随着镉染毒时间的延长而愈趋明显；肌动蛋白结构的改变先于形态学变化；在肌动蛋白改变的同时伴有细胞内ＡＴＰ水平的降低，随着染毒时间延长而逐渐加重。当镉被洗脱后，细胞内ＡＴＰ水平开始上升，而肌动蛋白分布并未出现明显回复现象。结论：镉染毒可导致细胞内能量代谢抑制，肌动蛋白结构改变，从而导致细胞结构及功能异常。肌动蛋白结构改变在重金属镉所致的细胞形态。bjective:To study the relationship between cellular energy, cytoskeleton and morphogic changes of renal tubular cells treated with cadmium. Methods: Observed the renal tubular cells in regular time course by immunofluorecence and ultrastructure technique. Results: It showed:① The structure integrity of F-actin of the renaltubular cells was changed very quickly in early of the injury course, and the change became significantly with prolong of perfusion time of cadmium;②The disruption of F-actin occurs early than morphologic change of tubular cells, and compared with a decrease of cellular ATP level by timededpendent; ③ The structure integrity of F-actin had not recovered after washing out of cadmium and a increase of cellular ATP. Conclusion: Cadmium may inhibit the metabolism of cellular energy and cause thedisruption of structure integrity of F-actin, and result in renal tubular apical structural and functional alterations. F-actin structurand disruption may play an important role in the pathophysiology of tubular cells injury by cadmium.

关 键 词：镉 肾小管上皮细胞 肌动蛋白 ATP 镉中毒 

分 类 号：R595.2[医药卫生—内科学]