原卟啉钠体内外抗乙肝病毒作用研究  

The Anti-HBV Effect of Protoporphyroin Disodium in Vitro and in Vivo

作　　者：范巧云[1] 刘群红[1] 王建[1] 

机构地区：[1]安徽理工大学医学院,安徽淮南32001

出　　处：《时珍国医国药》2008年第8期1858-1859,共2页Lishizhen Medicine and Materia Medica Research

基　　金：安徽理工大学硕士基金资助项目(No.2007Yb48);安徽理工大学青年教师基金项目(No.QN200644)

摘　　要：目的观察原卟啉钠体内外抗乙肝病毒作用。方法体内实验采用先天感染鸭乙肝模型,检测给药前、给药后5 d、10天及停药后3 d鸭血清DHBV-DNA的动态变化;体外实验采用2.2.15细胞为模型,检测用药后对细胞培养上清液中HBsAg和HBeAg的抑制作用。结果体内实验显示,给药第5,10 d时鸭血清DHBV-DNA其OD值均明显低于给药前,差异有显著性意义(P<0.05);体外实验显示,原卟啉钠在所稀释的各浓度下,对细胞分泌HBsAg,HBeAg均有一定的抑制作用,对HBsAg,HBeAg的治疗指数分别大于188.7和64.5。结论原卟啉钠体内外均具有明显的抗乙肝病毒作用。Objective To observe the inhibitory effect of NAPP on Hepatitis B virus in vitro and in vivo. Methods Duck model of congenital infection of HBV was used in the in vivo test. DHBV - DNA in serum was detected by dot - hybridization test before treatment, 5 days and 10 days after treatment. The ceil 2.2.15 line was taken as ceil model. The inhibitory effect of NAPP on HBsAg and HBeAg secreted by 2.2.15 ceils was evaluated with ELISA method. Results In the animal model, the optic density value of DHBV - DNA in duck serum was reduced markedly after treatment with NAPP for 5 days and 10 days, there was remarkable difference （P 〈 0.05 ）. In the cell model, NAPP had obvious inhibitory effect on HBsAg and HBeAg in different concentrations. The therapeutic index of NAPP was greater than 188.7 for HBsAg and 64.5 for HBeAg. Conclusion NAPP has a certain inhibitory effect on Hepatitis B virus in vitro and in vivo.

关 键 词：原卟啉钠 鸭乙型肝炎病毒 2.2.15细胞株 

分 类 号：R256.4[医药卫生—中医内科学] R285.5[医药卫生—中医学]