高效液相色谱法测定葛根、红花和山楂混合提取物中葛根素、羟基红花黄色素A的含量  

Determination of Puerarin and HSYA in Extracts of Radix Puerariae,Fructus Crataegi and Carthamus tintorius by HPLC

作　　者：李桥[1] 宋恬[1] 王光忠[1] 刘焱文[1] 

机构地区：[1]湖北中医学院.中药资源与中药复方省部共建教育部重点实验室,湖北武汉430061

出　　处：《时珍国医国药》2008年第8期1860-1861,共2页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省武汉市科技局专项资金资助项目(No.20056005130001)

摘　　要：目的建立一种反相高效液相色谱方法，测定葛根、山楂、红花混合提取物中葛根素和羟基红花黄色素A的含量。方法采用Hypersil ODS4．6mm（i．．d．）×250mm，5μm，以0．2％（v／v）磷酸水溶液：甲醇：乙腈（81：16：3）为流动相，流速1．2ml／min，柱温25℃，检测波长分别为250，403nm，测葛根素和羟基红花黄色素A。结果葛根素和羟基红花黄色素A线性范围分别为0．032406-0．16208mg/ml和0．0294～0．147mg／ml；平均加样回收率分别为98．3％，98．0％；RSD分别为1．2％，1．6％。结论该方法快速、简便、准确，可为评价该提取物的质量提供依据。Objective： To establish an RP - HPLC method for the determination of the content of Puerarin and HSYA in the extracts from Radix Puerariae, Fructus Crataegi and Carthamus tintorius. Methods The HPLC system consisted of Hypersil ODS column（250 mm×4.6 mm,id 5 μm） with phosphoric acid （0.2% ） ： methanol ： acetonitrile （ 81 ： 16 ： 3 ） mixture as mobile phase, the flow rate was 1.2 ml/min, the detection wavelength was 250,403 nm respectively for determination of Puerarin and HSYA, and the column temperature was 25%. Results The linearity ranges of Puerarin and HYSA were in the range of 0. 032 406 0. 162 08mg/ml and 0. 0294 -0. 147mg/ml respectively, the average recoveries of adding sample were 98.3% and 98.0% ,the RSD（n =5） were 1.2% and 1.6% respectively. Conclusion The method is simple , fast and accurate. It can be used for the quality control of the extracts of Radix Puerariae, Fructus Crataegi and Carthamus tintorius.

关 键 词：葛根素 羟基红花黄色素A 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]