不同溶剂海芋提取物的体外抗肿瘤活性研究  

Anticancer Effects of Diffenent Solvent Extracts from Alocasia Macrorrhiza in Vitro

作　　者：赵俊[1] 

机构地区：[1]天津科技大学生物工程学院.天津市工业微生物重点实验室,天津300222

出　　处：《时珍国医国药》2008年第8期1865-1866,共2页Lishizhen Medicine and Materia Medica Research

基　　金：天津科技大学自然科学基金资助项目(No.20050205)

摘　　要：目的研究海芋不同溶剂提取物的抗肿瘤作用。方法体外抗肿瘤活性研究采用MTT比色法,靶细胞采用人肺癌细胞株A549、黑色素瘤细胞株B16、人胃腺癌细胞株BGC-823、神经肿瘤细胞株NG108-15。结果海芋醋酸乙酯提取物对A549,B16,BGC-823的IC50分别为94.6,541.9,629.5μg.m l-1,丙酮提取物对A549,B16的IC50分别为40.9,438.0μg.m l-1,而海芋的环己烷,石油醚,乙醇,水提物对各种细胞株的IC50剂量都比较大。海芋的丙酮和醋酸乙酯提取物分别在60 g.kg-1和36.5 g.kg-1的给药剂量下没有显示明显的急性毒性。结论醋酸乙酯和丙酮提取物有较好的细胞毒活性,没有明显的急性毒性,这两部分有较好的抗肿瘤前景。Objective To study the antieaneer effects of different solvent extracts from Alocasia macrorrhiza in vitro. Methods MTT method was used to detect the survival of tumor cells treated with diffenent solvent extract from Alocasia macrorrhiza in vitro, tumor cell A549, B16, BGC - 823, NG108 - 15 was used as target cell. Results The IC50 of the ethyl acetate extract from Alocasia macrorrhiza to A549, B16, BGC -823 were 94.6, 541.9,629.5 μg·ml^-1 respectively,and those of the acetone extract from Alocasia macrorrhiza to A549 and B16 40.9,438.0μg·ml^-1 respectively. But there were quite large dosages of the IC50 of the extracts of cyclohexane, benzine, ethyl alcohol and water from Alocasia macrorrhiza to all kinds of cell lines. The obvious acute toxicities were not observed for the ethyl acetate and acetone extracts of Alocasia maerorrhiza under the treatment doses of 60g ·kg^-1 and 36.5 g·kg^ -1 respectively. Conclusion There is good cytotoxicity of the ethyl acetate and acetone extracts from Alocasia macrorrhiza to tumor cells, and no obvious acute toxicity has been observed. Therefore these two parts have good anti - tumor prospects.

关 键 词：海芋 提取物 抗肿瘤活性 

分 类 号：R285.5[医药卫生—中药学]