砂仁及其混淆品的高效液相色谱指纹图谱鉴别研究  

Identification of Amomum villosum and its Adulterants by HPLC Fingerprint

作　　者：王祥培[1] 吴红梅[1] 赵杨[2] 孙宜春[1] 靳凤云[1] 万德光[3] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]四川大学华西药学院,四川成都610041 [3]成都中医药大学药学院,四川成都610075

出　　处：《时珍国医国药》2008年第8期1967-1968,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立砂仁及其混淆品的高效液相色谱(HPLC)指纹图谱鉴定方法。方法采用RP-HPLC(DAD)法,梯度洗脱,测定阳春砂、海南假砂仁及艳山姜的指纹图谱,并作相似度比较分析。结果砂仁及其混淆品的HPLC指纹图谱有明显差异。结论HPLC指纹图谱具有重现性好,特征性强,方法简便等特点,可用于砂仁与其混淆品药材的鉴别。Objective To establish HPLC fingerprint for the identification of Amomum villosum and its adulterants. Methods Chromatographic fingerprint of Amomum villosum, Amomum chinense and Alpinia zerumbet was determined by RP - HPLC （DAD） and the gradient elution mode was applied in chromatographic separation, data were analyzed by Fingerprint Similarity Evalution Software to compare the similarity of samples. Results There was an apparent difference in fingerprint between Amomum villosum and its adulterants. Conclusion HPLC fingerprint method is repeatable and feasible and can be suitable for identifying Amomum villosum and its adulterants.

关 键 词：砂仁 混淆品 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]