SPIO标记的反义探针磁学性能检测  

Magnetic properties measurement of antisense oligodeoxynucleotide probes labeled by superparamagnetic iron oxide

作　　者：文明[1] 柏玮[2] 李必波[3] 韩忠[4] 陆云峰[1] 李少林[2] 杨学恒[4] 

机构地区：[1]重庆医科大学附属第一医院放射科,重庆400016 [2]重庆医科大学基础医学院放射医学教研室,重庆400016 [3]重庆医科大学药学院,重庆400016 [4]重庆大学数理学院,重庆400030

出　　处：《重庆大学学报（自然科学版）》2008年第8期956-960,共5页Journal of Chongqing University

基　　金：国家自然科学基金资助项目(30070230);重庆市卫生局医学科研资助项目(062025);重庆医科大学优秀博士基金资助项目(重医大文2006-182)

摘　　要：采用化学交联法制备超顺磁性氧化铁(SPIO)标记的c-erbB2癌基因反义脱氧寡核苷酸探针,经过1.5T磁共振仪测定,其T2弛豫率为0.156×106mol-1.s-1;振动样品磁强计测定出探针的磁化强度为69.4238 emu/g Fe,比饱和磁化强度68.4134 emu/g,比剩余磁化强度为30.3541 emu/g,剩磁为19.7345 Gs。为了检测探针的有效性,进一步使用该探针转染高表达c-erbB2癌基因的SK-Br-3肿瘤细胞株,同时使用正义和无义探针转染SK-Br-3肿瘤细胞株、反义探针转染正常小鼠肝细胞作对照,结果发现反义探针能够特异性进入SK-Br-3肿瘤细胞内,且能明显改善该细胞的磁学参数并降低磁共振扫描下的信号强度。The c-erbB2 oncogene antisense oligodeoxynucleotide probe was obtained by chemical cross-linking approach. Its T2 relaxivity was 0. 156 × 106 mo 1^-1· s^-1 , which was determined with a 1.5T MR system. The saturated magnetization, specific saturated magnetization, specific retentivity and retentivity were 69. 423 8emu/g Fe, 68. 413 4e mu/g, 30. 354 le mu/g and 19. 734 5 Gs, respectively. These measurements were obtained with a vibrating-sample magnetometer. To test its effectiveness, highly expressive SK-Br-3 oncocytes were transfected with the antisense oligodeoxynucleotide probe. Meanwhile, SK-Br-3 oncocytes were transfected with the sense and non-sense oligodeoxynucleotide probe, and normal mouse liver cells were transfected with the antisense oligodeoxynucleotide probe. The results show that the antisense probe could enter the SK-Br-3 oncocytes effectively, and improve the magnetic properties and decrease the signal intensity in MR scanning obviously.

关 键 词：分子影像学 反义脱氧寡核苷酸 氧化铁钠米 磁学参数 磁共振 细胞成像 

分 类 号：O482.5[理学—固体物理]