咳喘宁对支气管哮喘大鼠肺组织白细胞介素-5mRNA表达的影响  

Effect of Kechuanning on Expression of IL-5mRNA in Lung Tissue of Bronchial Asthmatic Rats

作　　者：杨牧祥[1] 于文涛[1] 徐华洲[1] 王晓红[2] 

机构地区：[1]河北医科大学中医学院,河北石家庄050091 [2]河北医科大学第二医院,河北石家庄050000

出　　处：《中国中医药信息杂志》2008年第9期34-36,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：河北省科技攻关计划(06276102D-37)

摘　　要：目的观察咳喘宁对支气管哮喘大鼠肺组织白细胞介素-5(IL-5)mRNA表达的影响。方法40只SD大鼠随机分为正常组、模型组、咳喘宁高剂量组(27g原药材/kg体重)、咳喘宁低剂量组(13.5g原药材/kg体重)、桂龙咳喘宁对照组(0.41g/kg体重),每组8只。除正常组外,以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每日灌胃给药,激发并给药4周后处死大鼠。采用RT-PCR检测IL-5mRNA表达,采用HE染色观察肺和气管病理组织学变化。结果与正常组比较,模型组大鼠支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织IL-5mRNA表达明显增加(P<0.01);与模型组比较,各治疗组均可显著降低支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及IL-5mRNA表达(P<0.05或者P<0.01)。结论咳喘宁可降低肺组织IL-5mRNA的表达,抑制嗜酸性粒细胞在气道的浸润、聚集和活化,减轻哮喘气道炎症。Objective To observe effect of Kechuanning on the expression of IL-5mRNA in lung tissue of bronchial asthmatic rats. Methods Forty rats were randomly divided into 5 groups： the normal group, the model group, Kechuanning high-dose group （27 g/kg）, Kechuanning low-dose group （13.5 g/kg）, Guilong Kechuanning control group （0.45 g/kg）, 8 rats in each group. The bronchial asthmatic model was established by egg protein sensibilization and inhalation provocation. The rats of each treatment group were lavage administration each day from the first time of provocation to execution. After 4 weeks of treatment, the rats were ldlled and lung tissue were taken to dying of HE to be observed. The expression of IL-5mRNA in lung tissue were determined by RT-PCR. Result Compared with the normal group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granulocyte and leukomonocyte, and expression of IL-5mRNA in lung tissue were increased （P〈0.01）. Compared with the model group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granulocyte and leukomonocyte, the expression of IL-5mRNA in lung tissue were decreased in each treatment group （P〈0.05 or P〈0.01）. Conclusion Kechuanning could decrease the expression of IL-5mRNA inhibit the infiltration, aggregation and activation of EOS and restrain air passage inflammation.

关 键 词：咳喘宁 支气管哮喘 白细胞介素-5 大鼠 

分 类 号：R289.11[医药卫生—方剂学]