BK8644对河豚毒素引起的大鼠海马锥体神经元AMPA受体介导的微小兴奋性突触后电流频率和幅度的影响  

Effect of BK8644 on frequency and amplitude of mEPSC_(AMPA) induced by tetraodontoxin in cultured hippocampal pyramidal neurons of rats

作　　者：揭艳玲[1] 李树基[1] 行艳丽[1] 龚芝[1] 朱心红[1] 高天明[1] 

机构地区：[1]南方医科大学神经生物学教研室,广东广州510515

出　　处：《新乡医学院学报》2008年第5期433-435,共3页Journal of Xinxiang Medical University

基　　金：国家自然科学基金(编号:30672660);广东省高等学校自然科学研究重点项目(编号:06Z007)

摘　　要：目的观察L-型钙通道(LTC)开通剂BK8644对河豚毒素(TTX)引起的大鼠海马锥体神经元α-氨基-羟基-5-甲基-4-异唑-丙酸(AMPA)受体介导的微小兴奋性突触后电流(mEPSCAMPA)的影响,探讨LTC是否作为神经环路兴奋性的感受器在调节突触稳态中发挥作用。方法建立培养海马神经元的稳态可塑性离体模型,并采用膜片钳全细胞模式记录突触内自发的mEPSC。结果TTX组mEPSCAMPA的频率显著高于对照组(P<0.05),而mEPSCAMPA电流幅度无显著差异(P>0.05);BK8644组和TTX+BK8644组与对照组比较,mEPSCAMPA的频率和幅度均无显著差异(P>0.05);TTX+BK8644组与TTX组比较,mEPSCAMPA频率降低(P<0.05),电流幅度无显著差异(P>0.05)。结论BK8644可对抗TTX引起的mEPSCAMPA频率升高,提示L-型钙通道可能参与稳态突触可塑性的调节。Objective To examine the effects of the L-type calcium channel agonist BK8644 on the mEPSCAMPA induced by tetraodontoxin （TTX） in cultured hippocampal pyramidal neurons of rats. and to explore the roles of L-type calcium channel in the regulation of homeostatic synaptic plasticity. Methods The model of homeostatic synaptic plasticity of cultured hippocampal pyramidal neurons was established, and the synaptic mEPSC was recorded by Whole-cell patch-clamp recordings. Results The frequence of mEPSCAMPA was higher in TTX group than this in control group（ P 〈 0.05 ） .but there was no significant difference in the amplitude of mEPSCAMPA between two groups（P 〉0.05 ）. There was no significant difference in the frequence and amplitude of mEPSCAMPA among BK8644 group, 333（ ＋ BK8644 group and control group（ P 〉0.05 ）. The frequence of mEPSCAMPA was lower in 133（ ＋ BK8644 group than that in 133（ group（ P 〈 0.05 ）. but there was no significant difference in the amplitude of mEPSCAMPA between two groups（ P 〉 0.05）. Conclusion BK8644 can prevent the increase of the frequence of mEPSCAMPA induced by TTX it suggest that the L-type calcium channel may be involved in the regulation of homeostatic synaptic plasticity.

关 键 词：L-型钙通道 海马神经元 膜片钳记录 微小兴奋性突触后电流 

分 类 号：R338.8[医药卫生—人体生理学]