好氧反硝化细菌N6-1亚硝酸盐还原活性的研究  

Study on the Nitrite-reducing Activity of Aerobic Denitrifying Bacterial Strain N6-1

作　　者：陈松[1] 洪秀娟[1] 黄雷鸣[1] 窦洁[1] 周长林[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009

出　　处：《微生物学通报》2008年第7期1007-1010,共4页Microbiology China

摘　　要：针对本实验室筛选得到的1株好氧反硝化细菌(Aerobic Denitrifying Bacteria)N6-1,研究了培养条件和培养基组分对菌株亚硝酸盐还原活性的影响。结果表明,其最适培养条件为30℃、摇床转速120r/min、初始pH8.5,分别以NaNO2和乙酸钠为唯一氮源和碳源时,最适碳氮比为12;初始NaNO2浓度为2g/L时,培养20h后NO2??N全部被还原,平均还原速率可达20.3mg/L·h;1.5%NaCl和1%蛋白胨对其亚硝酸盐还原活性没有明显影响;10L发酵罐培养24h后菌体浓度高达1.2×1011CFU/mL。The nitrite-reducing activity of aerobic denitrifying bacterial strain N6-1 was studied. It showed that the nitrite-reducing activity reached the highest at 30℃, 120 r/min, pH 8.5 and C/N ratio 12, using CH3COONa and NaNO2 as the sole carbon source and nitrogen source, respectively. When the initial NaNO2 concentration was 2 g/L, NO2-N was reduced completely after 20 hours cultivation with the reducing rate of 20.3 mg/L.h. There would be no effect on its nitrite-reducing activity in the present of 1.5% NaCl or 1% peptone. The cell concentration could reach 1.2× 10^11 CFU/mL after 24 hours cultivation in 10 L fermentor

关 键 词：好氧反硝化细菌 亚硝酸盐还原 还原速率 发酵罐 

分 类 号：X703[环境科学与工程—环境工程]