黑芝菌丝体液体培养基的筛选  

Selection of liquid culture media on mycelium of Ganoderma atrum

作　　者：廖伟[1] 郭成金[1] 

机构地区：[1]天津师范大学化学与生命科学学院,天津300387

出　　处：《天津师范大学学报（自然科学版）》2008年第3期8-10,26,共4页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市科学技术委员会项目(06ZHCXNC05900)

摘　　要：以黑芝菌丝体生物量为主要指标,首先对不同碳源和氮源进行单因素初选试验,然后进行不同比例混合碳氮源正交试验.结果表明:黑芝菌丝体液体最佳培养基是蔗糖20 g/L、麦芽糖15 g/L、麦麸20 g/L、酵母粉1.5 g/L、KH2PO42 g/L、MgSO4.7H2O 1 g/L和VB16 mg/L,其菌丝体生物量产率为35 g/L.Using mycelium biomass of Ganoderma atrum in liquid culture media as an major index,different carbon source and nitrogen source were added into liquid culture media to carry out the single factor primary election,then a orthogonal experiment was carried out with different proportional commixture of carbon source and nitrogen source.The result showed that the best medium was cane sugar 20 g/L,maltos 15 g/L,wheat bran 20 g/L,yeast extracts 1.5 g/L,KH2PO4 2 g/L,MgSO4·7H2O 1 g/L and VB1 6 mg/L,and the mycelium biomass of Ganoderma atrum reached 35 g/L in the liquid culture medium.

关 键 词：黑芝 液体培养基 筛选 菌丝体 生物量 

分 类 号：Q93-335[生物学—微生物学]