加味青麟丸对老龄大鼠抗氧化作用的影响  

Effect of Jiaweiqinglin Pills on antioxygenation in aged rats

作　　者：佘延芬[1] 杨继军[1] 杨阳[1] 张瑞娟[2] 杨云峰[2] 

机构地区：[1]河北医科大学中医学院,河北050091 [2]河北医科大学研究生学院

出　　处：《北京中医药大学学报》2008年第7期467-469,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：河北省科技厅基金资助项目(No.03276196D-44);河北医科大学青年科技基金项目(No.030018)

摘　　要：目的通过观察加味青麟丸对老龄大鼠抗氧化的作用,探讨该药抗衰老的相关机制。方法选用Wistar大鼠120只,随机分为6组,即4月龄空白对照组(4C组)、14月龄空白对照组(14C组)、14月龄加味青麟丸组(14 JL组)、24月龄空白对照组(24C组)、24月龄加味青麟丸组(24 JL组)和14-24月龄加味青麟丸组(14-24 JL组),每组各20只,雌雄各半。按实验设计要求喂养,各组于相应月份剖杀后测定血清和心、肝、肾组织超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量及血清GSH-PX活力。结果对比14C组大鼠,该药可显著提高14 JL组大鼠血清、肝组织中SOD活力,及其血清GSH-PX的活力;可显著降低血清、心肌组织、肝组织中MDA含量。对比24C组大鼠,该药可显著提高24 JL组与14-24 JL组大鼠血清、心肌组织、肝组织、肾组织中SOD活力,及其血清GSH-PX的活力;显著降低血清、心肌组织、肝组织、肾组织中MDA含量。结论加味青麟丸可提高老龄大鼠抗氧化能力。Objective To observe the effect of Jiaweiqinglin Pills（JP） on antioxygenation in aged rats to investigate the its antidotal mechanism.Method After adaptability feeding for one week,120 healthy Wistar rats were randomly divided into 6 groups,which were 4-month control group（4C group）,14-month control group（14C group）,14-month JP group（14JP group）,24-month control group（24C group）,24-month JP group（24JP group） and（14-24）-month JP group（14-24JP group）,with 10 males and 10 females in each group.All rats were put to death after the last time medication and fasting for 12 hours.SOD and MDA in serum and in the tissues of heart,brain and liver were detected,and glutathione peroxidase（GSH-PX） activity in serum was measured.Results Vitality of SOD in serum and liver tissue of 14C group is lower than those of 4C group（P〈0.01）and 14JP group（P〈0.01）.But it was not obvious in the heart and kidney tissues.Contents of MDA in serum and in the heart and liver tissue of 14C group were higher than those of 4C group（P〈0.01）and 14JP group（P〈0.05）.Vitality of GSH-PX in serum of 14C group is lower than it of 4C group（P〈0.01）and 14JL group（P〈0.01）.Vitality of SOD in serum,heart liver and kidney tissue of 24C group is lower than it of 24JL group（P〈0.01）and 14-24JL group（P〈0.01）.To the best obvious is the vitality of SOD in serum..Vitality of GSH-PX in serum of 24C group was lower than that of 24JP group（P〈0.01）and 14-24JP group（P〈0.01）.Contents of MDA in serum and in the heart,liver and kidney tissues of 24C group were higher than those of 24JP group（P〈0.01）and 14-24JP group（P〈0.05）,especially in the serum.The MDA of kidney of 24JP was lower than that of 14-24JP.Conclusion Jiaweiqinglin Pills may enhance the antioxidation mechanism of aged rats.

关 键 词：加味青麟丸 老龄大鼠 自由基 抗氧化 

分 类 号：R285.5[医药卫生—中药学]