电针对急性脊髓损伤大鼠差异表达基因及钙离子作用的实验研究  

Influence of electroacupuncture on genes and calcium ion in rats with acute spinal cord injury

作　　者：李志刚[1] 刘如春[1] 耿直[2] 张春燕[1] 

机构地区：[1]北京中医药大学针灸学院,北京100029 [2]新疆医科大学

出　　处：《北京中医药大学学报》2008年第7期486-489,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局资助项目(No.04-05JP02)

摘　　要：目的探讨电针对急性脊髓损伤(SCI)保护作用的机理。方法大鼠随机分为空白组、假手术组、模型组、药物组、电针组,用改良Allen s打击法制成SCI模型。电针组造模后电针"大椎""命门"两穴,药物组尾静脉注射甲基强的松龙。损伤6 h后取材。测定损伤后血清中钙离子含量;取电针组、模型组和空白组的损伤局部组织,提取mRNA,由空白组、模型组和电针组脊髓标本中提取的RNA样品中,各选取3份RNA样品,分别混合后组成具有可比性的2组获得差异表达基因,通过生物信息学方法对这些差异表达基因在急性SCI中的作用进行功能分析。结果与模型组相比,电针组钙离子含量降低显著(P<0.05)。SCI的早期,模型-空白组有266条基因的表达发生了显著变化;电针-空白组有181条差异表达基因。结论电针可以降低SCI大鼠血清中钙离子含量,对SCI具有保护作用;脊髓损伤后有大量的基因差异表达发生,这些基因可能与神经组织保护、生长和再生关系密切。Objective To discuss the protective mechanism of electroacupuncture（EA） in the acute spinal cord injury（SCI）.Methods Rats were divided into five groups stochasticly： blank group,sham operated group,model group,drug group and EA group.The model rats were made a spinal cord contusion by using a modified Allen＇s method.After SCI the EA group was treated by ＂mingmen＂（DU4） and ＂dazhui＂（DU14） points;the drug group was treated by methylprednisolone（MP）.Six hours after SCI,the rats were put to death.The calcium ion content in serum was detected and the differentially expressed genes were investigated by bioinformatics technique.Results The calcium ion content in serum of the EA Group decreased significantly as compared with those in the model group（P〈0.05）.There were 266 differentially expressed genes in the model group and 181 differentially expressed genes in the EA group.Conclusion EA had protective effect on the SCI through depressing the calcium ion content.There were lots of differentially expressed genes in SCI rats.These differentially expressed genes are relative with protective effect,growth and regeneration of the nerve tissues.

关 键 词：电针 脊髓损伤 钙离子 基因 大鼠 

分 类 号：R245.31[医药卫生—针灸推拿学]