甜菜碱对小鼠脾淋巴细胞的增殖作用  

Effects of betaine on mouse splen-lymphocytes in vitro

作　　者：季宇彬[1] 冯小燕[1] 高世勇[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站

出　　处：《哈尔滨商业大学学报（自然科学版）》2008年第4期385-388,395,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(30400352);教育部重点项目(205045);黑龙江省自然科学基金(D200611);黑龙江省研究生创新基金(YJSCX2006-0077HSD)

摘　　要：研究甜菜碱对小鼠脾淋巴细胞的增殖作用.应用MTT法观察体外甜菜碱在不同浓度、不同时间对小鼠脾淋巴细胞增殖的影响,应用流式细胞仪(FCM)测小鼠脾淋巴细胞在不同时间周期的变化.结果表明,甜菜碱终浓度0.4、4、20 mmol/L均可促淋巴细胞增值,但终浓度4 mmol/L效果最为明显,在甜菜碱刺激24、48、72 h后,淋巴细胞均可显著增值,但随时间的加倍,增值幅度并不明显,综合考虑,24 h效果更好.终浓度4 mmol/L甜菜碱不同时间均可促进淋巴细胞由G0/G1期进入S期,且作用18 h效果最明显,其G0/G1期由97.03%下降到89.99%,S期由2.58%升高到9.08%.到24 h后,其诱导作用反而有些下降.因此,甜菜碱4 mmol/L作用24 h促淋巴细胞增殖最为理想.4 mmol/L的甜菜碱18 h促进淋巴细胞由G0/G1期进入S期的作用效果最好.To investigate the effects of betaine on splen-lymphocytes proliferation, observe the effects of the betaine on the lymphocyte proliferation of mouse by MTT at different con- centrations and times. To observe the effects of the betaine on the lymphocyte proliferation cell-cycle of mouse by FCM. The result indicate that betaine can stimulate lymphocytes proliferation at the concentrations of 0. 4 mmol/L,4 mmol/L and 20 mmol/L, but 4 mmol/L is the best; betaine can stimulate lymphocytes proliferation at the time of 24 h,48 h and 72 h, but 24 h is the best. Betaine can change the cell - cycle distribution of lymphocytes at different times, and the best time of increasing the proportion of S-phase cells is 18 h , rai-sing from 2. 58% to 9. 08%. Betaine can stimulate lymphocytes proliferation and the best concentrations is 4 mmol/L, the best time is 24 h. Betaine can increase the proportion of Sphase cells, 18 h is the best.

关 键 词：甜菜碱 淋巴细胞增殖 细胞周期 

分 类 号：R285[医药卫生—中药学]