野西瓜挥发油诱导SGC-7901凋亡的初步研究  

Study on mechanism of total oil in capparis spinosa inducing apoptosis in SGC-7901 cells

作　　者：王崴[1] 于蕾[1] 崔荣田[1] 莫科[1] 邹翔[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站

出　　处：《哈尔滨商业大学学报（自然科学版）》2008年第4期396-399,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省自然科学基金项目(D200610)

摘　　要：研究野西瓜挥发油诱导SGC-7901细胞凋亡的机制.采用流式细胞术分析野西瓜挥发油对SGC-7901细胞早期凋亡的影响;采用激光扫描共聚焦技术观测野西瓜挥发油对肿瘤细胞内Ca2+浓度变化的影响;采用流式细胞术分析野西瓜挥发油对SGC-7901细胞线粒体跨膜电位的影响.野西瓜挥发油可以诱导SGC-7901细胞发生早期凋亡,使肿瘤细胞内的Ca2+浓度升高,并降低细胞线粒体跨膜电位.野西瓜挥发油具有明显的抗肿瘤作用,其抗肿瘤机制为诱导肿瘤细胞凋亡.通过升高肿瘤细胞内的[Ca2+]i而激活了Ca2+依赖性的核酸内切酶,同时通过PT孔的开放,引起线粒体跨膜电位下降而导致细胞凋亡.To study the mechanism of oil inducing apoptosis in SGC-7901 cells. The apoptosis in the initial stage of SGC-7901 cells is studied by flow cytometry; the effects on [Ca^2＋] i of human gastric carcinoma cells is observed by confocal laser scanning technique. The electric potential of mitochondrion is studied by flow cytometry. The apoptosis in the initial stage of SGC-7901 cells, the advance of [Ca^2＋] i of human gastric carcinoma cells and the loss of electric potential of mitochondrion could be induced by total oil in Capparis spinosa. Total oil has significant anti-tumor effect. The mechanism of anti - neoplastic action, inducingapoptosis in SGC -7901 cells is mechanism of total oil in Capparis spinosa.

关 键 词：野西瓜 挥发油 细胞凋亡 [CA^2+]I 

分 类 号：R285[医药卫生—中药学]