三维丝素蛋白/羟基磷灰石支架的生物学性能  

In vitro study on biological characteristics of 3D non-woven silk fibroin net/nano-hydroxapatite scaffold

作　　者：赵勇[1] 曾泉[2] 陈静[1] 陈治清[1] 

机构地区：[1]四川大学华西口腔医学院口腔材料学研究室,成都610041 [2]四川大学纳米生物医学技术与膜生物学研究所,成都610041

出　　处：《四川大学学报（自然科学版）》2008年第4期859-863,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家863课题(2002AA326080)

摘　　要：综合运用丝素蛋白纤维网的非编织制作方法和仿生矿化法,构建三维多孔丝素蛋白/纳米羟基磷灰石复合支架.通过体外蛋白酶降解和成骨细胞培养,对支架材料的生物学性能进行初探.发现蛋白酶对丝素蛋白降解的作用大小依次为,蛋白酶K>胰蛋白酶>α-糜蛋白酶>Ⅰ型胶原酶.所构建的支架显示出良好的生物相容性并能促进成骨细胞生长和分化.三维多孔丝素蛋白/纳米羟基磷灰石支架可望成为新型有机/无机复合生物材料,为骨组织工程支架的设计提供了新的思路.In vitro experiments were carried out to investigate the enzymatic degradation of the scaffold. Osteoblast cultivation was tested by SEM, MTT and ALP to evaluate the biological reaction of the scaffold. It is laud that, silk fibroin is biodegradable. Enzymatic degradation of fibroin indicated that the extent of weight loss depended on the type of enzyme, the enzyme-to-substrate ratio, and the treatment time. Among the enzymes, protease K was the most aggressive and followed by trypsin, α-chymotrypsin and collagenase I. The protease K solution with the concentration of 3 × 10^-4 g/mL almost caused a complete weight loss within 20 days of incubation. NSFN/nHAP showed an excellent cytocompatibility for the growth of osteoblasts and had the capabilities to improve the viability of osteoblasts. Porous NSFN/nHAP scaffold may be a hopeful scaffold used in bone tissue engineering.

关 键 词：骨组织工程 仿生矿化 细胞培养 降解 丝素蛋白 

分 类 号：TQ46[化学工程—制药化工]