抗—BudR单克隆抗体制备及特性鉴定  

PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO BROMODEOXYURIDINE

作　　者：胡有秋[1] 除主初 陈卫三 彭林 段朝军[1] 肖志强[1] 

机构地区：[1]湖南医科大学肿瘤研究所

出　　处：《湖南医科大学学报》1990年第4期387-390,共4页Bulletin of Hunan Medical University

基　　金：国家自然科学基金

摘　　要：用碘化还原法将溴脱氧尿嘧啶(BudR)与牛r—球蛋白(BGG)偶联作为抗原(BudR—BGG)免疫BALB/c小鼠,取脾细胞与小鼠骨髓瘤细胞(653)进行融合,经放射免疫分析法(RIA)筛选和克隆化后,获得四株阳性杂交瘤Ⅱ_1B_7、Ⅱ_4B_4、Ⅰ_3F_(11)和Ⅱ_1H_7。杂交瘤细胞分别腹腔注射BALB/c小鼠,其中三株(Ⅱ_1B_7、Ⅱ_4B_4和Ⅱ_1H_7)获得腹水,纯化抗体并对其特性进行鉴定,三株单抗均可与BudR、IudR反应,而不与CdR、TdR及FudR反应,具有较高的特异性。Preparation and characteristics of three mouse monoclonal antibodies specific for 5-bromod-eoxyuridine(BudR)is presented. Three hybridomas, Ⅱ 1H7, Ⅱ 4B4 Ⅱ1B7,were derived from the fusion of spleencells from BALB/c mouse immunized with BudR bovine gamma globulin (BGG)conjugate and '653 mouse myeloma'cells. Enzyme-linked immunohistochemistry against whole cells labeled with BudR and radioimmunologic assay(RIA)showed that:all. three antibodies have similar binding affinities and specifities. The antibodies react to BudR specifically but do not recognize CdR,FudR or TdR.

关 键 词：单克隆抗体 细胞动力学 肿瘤 

分 类 号：R392－33[医药卫生—免疫学]