胶原诱导型关节炎大鼠的关节影像学特点  

Radiographic Imaging Features of Collagen-Induced Arthritis in Rats

作　　者：陈光星[1] 童娟[1] 周伟生[1] 黄勇[1] 刘清平[1] 陈纪藩[1] 

机构地区：[1]广州中医药大学第一附属医院六内风湿专科,广州510405

出　　处：《中国实验动物学报》2008年第4期244-249,I0001,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金重点项目资助(编号:39930230);广东省中医药管理局课题资助(编号:102019)

摘　　要：目的旨在分析CIA X线片四肢关节的破坏特点,揭示CIA大鼠关节破坏的规律,为规范评分方案提供依据。方法采用П型胶原和弗氏完全佐剂皮下注射清洁级Wistar大鼠,造模成功(每批10只,共3次)后第35天行全身X线钼靶照片,以正常组作为对照、每只大鼠评价96块骨破坏(erosion)和100个关节间隙(joint space narrowing,JSN);处死动物,取左前肢和右后肢近端第3足趾关节苏木素-伊红(HE)染色,评价中性粒细胞、淋巴细胞、浆细胞浸润、滑膜增生和软骨破坏的情况。结果造模成功后CIA大鼠关节出现明显的红肿,活动受限;HE病理显示,CIA关节存在明显的中性粒细胞、淋巴细胞和浆细胞浸润,滑膜增生,纤维组织增生,软骨破坏;X线片分析结果显示:①广泛性骨质疏松,边缘性骨质侵蚀,关节间隙狭窄或增宽,部分踝关节间隙消失,关节相互融合甚至骨性强直。②67%的骨出现erosion,JSN影响为78%,关节破坏以中、重度为主;③远端、近端趾间关节和踝关节发病率高,损害严重,掌趾关节发病率低,破坏较轻。④后肢关节破坏重于前肢(P<0.01),左右肢没有显著性差异(P>0.05)。结论①滑膜是CIA炎症反应启动的主要病灶,与骨交界的滑膜和血管翳造成了CIA的骨质破坏;②CIA影像学表现关节破坏严重,以远端、近端趾间关节和踝关节为主,这些关节可作为评价破坏程度的选择。本研究对于深入CIA关节破坏的病因病理和进一步规范X线片评分方案具有一定意义。Objective Collagen-induced arthritis （CIA） in rats is a classical experimental model for the investigation of rheumatoid arthritis （RA） in humans. However, no criteria are available in the joint selection for the evaluation of the radiographic manifestations of CIA in rats. The purpose of this study was to conduct a systemic investigation on radiographic manifestations of CIA in rats, and to clarify the effect of selection of different joints on CIA assessment. Methods CIA was induced with bovine collagen Ⅱ in 30 Wistar rats. The onset of CIA was monitored by feet swelling and histological analysis. The radiographic manifestations in 30 rats with CIA were assessed on the bases of erosion of 96 joints and joint space narrowing （JSN） of 100 joints per rat. Results Rats with CIA showed distinct foot swelling and signs of severe arthritis along with infiltration of neutrophils, lymphocytes and plasmocytes, hyperplasia of synovial cells and fibrous tissue, and cartilage destruction at 35 days after immunization. X-ray films of joints in the four Iimbs revealed that rat CIA was characterized by symmetrical tissue swelling of joints, diffuse osteoporosis, bone erosion, exostosis of bone and abnormal joint spaces. About 67% and 78% of all joints showed erosion and JSN, respectively, soon after the onset of CIA and their incidence was highly correlated. The distal interphalangeal （DIPs）, proximal interphalangeals （PIPs） and ankle joints suffered from more severe damage, while metacarpaphalangeal and wrist joints were less affected. More severe destruction occurred in the joints of hindlimbs than those in forelimbs, but there was no difference in the severity of destruction between the left and right paws. Conclusion The joints with a high incidence rate and severe destruction, including the DIP, PIP and ankle joints in one-side hindlimbs, should be chosen in the evaluation of radiographic manifestations of CIA in rats.

关 键 词：胶原诱导型关节炎 X线片 关节破坏 

分 类 号：R543.13[医药卫生—心血管疾病]