不同时间脂肪乳输注对大鼠葡萄糖输注率的影响  

Effect of Intravenous Intralipid Infusion on Glucose Infusion Rate at Different Times in Rats

作　　者：何冰[1] 赵晟[1] 韩萍[1] 李淑文[1] 卢雁[1] 

机构地区：[1]中国医科大学附属盛京医院,沈阳110004

出　　处：《中国实验动物学报》2008年第4期298-300,共3页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的观察不同时间脂肪乳输注使血浆游离脂肪酸(FFA)升高对大鼠葡萄糖输注率(GIR)的影响。方法分别给大鼠输注脂肪乳2、5、7和48 h,行高胰岛素-正血糖钳夹试验评价葡萄糖输注率,测定血浆葡萄糖、FFA。结果与生理盐水输注组比较,2 h脂肪乳输注使血浆FFA升高了2倍(P<0.01),GIR下降27%(P<0.05),脂肪乳输注5 h GIR降低了52%(P<0.01),7 h GIR降低56%(P<0.01),输注48 h脂肪乳GIR降低58%(P<0.01),5 h组7、h组及48 h脂肪乳输注组间GIR差异没有统计学意义。结论大鼠输注脂肪乳使FFA浓度达到基础值的3倍左右,可复制出脂毒性胰岛素抵抗的模型,而且胰岛素抵抗达到一定程度后保持恒定。Objective To investigate the effect of intravenous intralipid infusion elevating the plasma free fatty acids （FFA） level at different times on glucose infusion rate in rats. Methods Intralipid ＋ heparin （IH） and saline （SAL） were intravenously infused for 2 h, 5 h, 7 h, and 48 h, respectively. Insulin sensitivity was assessed with glucose infusion rate （G/R） and determined by hyperinsulinemic-euglucemic clamp method. The levels of plasma glucose and FFA were measured. Results Compared with the SAL group, the plasma FFA level was elevated by 2 times （ P 〈 0.01 ） in group IH at 2 h, while GIR decreased 27% （ P 〈 0.05） at 2 h, decreased 52% at 5 h （ P 〈 0.01 ）, decreased 56% at 7 h （ P 〈 0.01 ）, and decreased 58 % at 48 h （ P 〈 0.01 ）, however there was no significant difference between the GIR values at 5 h, 7 h and 48 h. Conclusion A rat model of insulin resistance mediated by lipotoxicity has been successfully established by intravenous intralipid infusion, when the plasma FFA concentration reaches 3 times of the basic level and still maintaining such a high level for a longer time period.

关 键 词：游离脂肪酸 胰岛素抵抗 葡萄糖输注率 大鼠 

分 类 号：R977.15[医药卫生—药品]