Cu^(2+)对栉孔扇贝外套膜组织培养的影响  

Effect of Cu^(2+) on Mantle Tissue Culture of Chlamys farreri

作　　者：李清[1] 孙振兴[1] 张丽[1] 苏小珊[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《安徽农业科学》2008年第23期9882-9884,共3页Journal of Anhui Agricultural Sciences

基　　金：鲁东大学科研基金项目(L20073303)

摘　　要：[目的]了解重金属对栉孔扇贝的毒理效应。[方法]在DMEM培养液中分别添加质量浓度为0.05、0.10、0.20、0.40 mg/L的Cu2+,以细胞迁出时间和组织块增殖状况为评价指标,研究Cu2+对栉孔扇贝外套膜组织培养的影响。[结果]栉孔扇贝外套膜在DMEM培养液中贴壁良好。培养至72 h时,Cu2+质量浓度为0.05 mg/L的实验组有游离细胞迁出,且组织块增殖情况良好。培养至89 h时,Cu2+质量浓度为0.10 mg/L的实验组有游离细胞迁出,且组织块增殖情况较差。随着Cu2+质量浓度的增大,细胞迁出所需的时间越长,组织块增殖情况越差。Cu2+质量浓度为0.40 mg/L的实验组,培养96 h时并没有游离细胞迁出。[结论]Cu2+质量浓度大于0.10 mg/L时对栉孔扇贝外套膜的组织培养有一定的抑制作用。[ Objective ] The aim was to understand the toxicological effects of heavy metal on Chlamys farreri. [ Method ] Adding Cu^2＋ with conch, of 0.05, 0.10, 0.20, 0.40 mg/L into DMEM medium, the effect of Cu^2＋ on tissue culture of C. farreri mantle was investigated with cell emigration time and proliferative status of tissue blocks as evaluation index. [ Result] C. farreri mantle anchored well in DMEM medium.. When C. farreri mantle was cultured for 72 h, the experimental group with 0.05 mg/L Cu^2＋ had emigration of free cells and the proliferative status of tissue blocks was well. When C. farreri mantle was cultured for 89 h, the experimental group with 0.10 mg/L Cu^2＋ had emigration of free cells and the proliferative status of tissue blocks was poor. With the increase of Cu^2＋ conch. , the cell needed the longer migrating time and the proliferative status of tissue blocks was worse. The experimental group with 0.40 mg/L Cu^2＋ had no emigration of free cells When C. farreri mantle was cultured for 96 h. [ Conclusion] TheCu^2＋ with eonen, above 0.10 mg/L had some inhibitory effect on the tissue culture of C. farreri mantle.

关 键 词：栉孔扇贝 外套膜 CU^2+ 组织培养 

分 类 号：S917[农业科学—水产科学]