蚊净香草的离体快繁工艺  

Rapid Propagation Technique in vitro of Pelargonium odoratissmum

作　　者：刘学英[1] 

机构地区：[1]潍坊学院生物工程学院,山东潍坊261000

出　　处：《安徽农业科学》2008年第23期9893-9895,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究蚊净香草试管繁殖的诱导、分化、继代、生根、移栽等过程，筛选优良试管苗。[方法]以蚊净香草的侧芽、展开幼叶为外植体，以MS为基本培养基，加不同浓度的6-BA、IAA，进行外植体的启动培养。[结果]侧芽适宜的诱导培养基为6-BA0．2mg／L＋IAA0．1mg/L；叶片和叶柄的最佳诱导分化培养基为6-BA0．5～1．0mg／L＋IAA0．25～0．50mg/L；快速繁殖培养基以6-BA0．2mg／L＋IAA0．1131g／L最好；生根培养基以1／2MS＋NAA0．2mg／L效果较好；适宜的移栽基质为草炭：蛭石：珍珠岩=3：2：1。[结论]移栽时用保护性杀菌剂多菌灵等防止杂菌的滋生，可以有效提高移栽成活率。[ Objective] The research aimed to study the tube propagation process of induction,differentiation, subculture,rooting, transplanting of P. odoratissmum, and to screen fine tube seedlings. [ Method ] The lateral bud and spread young leaf of Pelargonium odoratissmum was used for explant,MS for basic medium, adding different contents 6-BA, TAA. Then the initiation culture of explant was carried out. [ Result ] The superior induction medium of lateral bud was 6-BA 0.2 mg/L ＋ IAA 0.1 mg/L. The superior inductive differentiation medium of leaf and petiole was 6-BA 0.5 - 1.0 mg/L ＋ IAA 0.25 -0.50 mg/L. The superior rapid propagation medium was 6-BA 0.2 mg/L ＋ IAA 0.1 mg/L. The superior rooting medium was 1/2MS ＋ NAA 0.2 mg/L. The suitable ratio of transplanting medium for peat：vermiculite：perlite was 3：2：1. [Conclusion] Protective fungicide such as earbendazim was used to inhahit mixed bacterium. And the rate of transplanting could be improved at the same time.

关 键 词：蚊净香草 离体快繁 工艺流程 

分 类 号：S603.6[农业科学—园艺学]