发状念珠藻多糖的提取及其对农作物种子发芽率的影响  

Extraction of Polysaccharide from Nostoc flagelliforme and Its Effect on the Germination Rate of Crop Seeds

作　　者：梁文裕[1] 马秀丽[1] 郑国旗[1] 谢亚军[1] 尹晓雯[1] 王星[1] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021

出　　处：《安徽农业科学》2008年第23期9944-9945,9981,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30760022);宁夏自然科学基金项目(B002-2004)

摘　　要：[目的]为发状念珠藻多糖的提取、开发及生物学活性研究提供试验及理论基础。[方法]以发状念珠藻为材料，用石油醚脱脂脱色素，80％的乙醇去可溶性小分子糖类，用水作提取剂，98℃下提取3次，每次3h，合并提取液后减压浓缩，三氯乙酸脱蛋白，SephadexG-50层析纯化，醇析后冷冻干燥，进行红外光谱测定和种子发芽试验。[结果]结果表明，发状念珠藻多糖得率为15．8％～19．7％，红外光谱的特征吸收峰明显，是一种含氨基和B糖苷键，不含硫酸基的多糖。当多糖浓度为1．0mg／L时油菜种子、小麦种子、水稻种子和胡麻种子的发芽率分别比对照组增加14．29％、12．79％、4．40％和15．48％，而多糖浓度为2．0mg／L时白菜和玉米种子的发芽率分别比对一照组增加11．24％和22．50％。[ Objective ] The research aimed to provide experimental and theoretical basis for the extraction and biological activities of polysac- charides from Nostocflagelliforme. [ Method ] Taking N. flagelliforme as the material, after refluxing with petroleum aether to remove fatty materials and pigments, 80% alcohol was used to remove dissolubility saccharide with lower molecular weight, extracting for 3 times with water at 98 ℃ for 3 h. The extracting solutions were concentrated under vacuum after blending raffinate, After protein removal by TCA, Sephadex G-50 chromatography purification was carried out, Seed germination test was conducted by infrared spectrum, [ Result] The results showed that the yield of polysaccharide was 15.8% - 19.7%. Characteristic absorption ＇peak of infrared spectrum was obvious. The substance was a kind of polysaccharide with -NH2 and IB-glycosidic linkage, but without -SO4. The average germination percentage of Brassica chinensis, Semen tritici aestivi, Oryza satival and Linum usitutissimum increased by 14.29 %, 12.79%, 4.40% and 15.48% , respectively, compared with the control when added 1.0 mg/L polysaccharides of N. flagelliforrne. While polysaccharides concentration being 2.0 mg/L, the germination rates of Brasslca campestris and Zea rnays enhanced by 11.24% and 22.50% , respectively.

关 键 词：发菜 多糖 提取 发芽率 生物学活性 

分 类 号：S330.21[农业科学—作物遗传育种]