用静息张力表征离体蟾蜍腓肠肌等长收缩能力  

Rest tension-based characterization of isometric contractility of Bufo gastrocnemius ex vivo

作　　者：廖飞[1] 郭睿[1] 李生兵[1] 左渝萍[2] 陆杰[3] 孙安平[3] 

机构地区：[1]重庆医科大学药学院重庆市生物化学与分子药理学重点实验室生物信息学与分子工程研究室,重庆400016 [2]重庆医科大学基础医学院生物化学与分子生物学教研室,重庆400016 [3]重庆医科大学基础医学院生理学教研室,重庆400016

出　　处：《南方医科大学学报》2008年第8期1412-1414,1418,共4页Journal of Southern Medical University

基　　金：重庆市高校首批优秀中青年骨干教师资助

摘　　要：目的考察用静息张力表征离体蟾蜍腓肠肌等长收缩能力的有效性。方法SOD抑制剂和维生素C联合作用蟾蜍腓肠肌,记录电刺激(12VDC,2ms波宽,2s间隔)下等长收缩张力变化,单指数函数加权拟合90%峰张力后的松弛过程测定松弛速度和静息张力表征收缩能力。结果收缩1.0min后对照静息张力在7.0min内单调下降而药物处理后静息张力先降后升,7.0min后处理组静息张力相对初始值的增加值显著高于对照组。同时,对照松弛速度大多单调下降,药物处理后松弛速度多数先升后降,且10.0min内其松弛速度总低于相同时刻的对照组。结论静息张力可反映肌肉收缩能力。Objective To characterize the isometric contractility of Bufo gastrocnemius ex vivo in light of the rest tension. Methods Bufo gastrocnemius treated with SOD inhibitor and ascorbate was stimulated electrically （12 V DC, 2 ms duration with a 2 s interval） to record the tension within 10 min. Weighted fitting to the relaxation curve of the tension below 90% of the peak tension with a mono-exponential model yielded the rest tension and relaxation rate. Results The control gastrocnemius showed monotonic decrease of the rest tension, but treatment with SOD inhibitor and ascorbate resulted in a decrease of the rest tension followed by a fast increase within a 1.0 min contraction. The increase of the rest tension at 7.0 min of contraction of the treated muscle was significantly greater than that of the control muscle. The control muscle showed a monotonic decrease of the relaxation rate in 10 min, whereas treatment with SOD inhibitor and ascorbate produced increased relaxation rate followed by monotonic decrease till a plateau was reached. In the course of the 10 min recording, the relaxation rate of the treated muscle was lower than that of the control after the same duration of contraction. Conclusion Rest tension is a characteristic index to represent the skeletal muscle contractility.

关 键 词：蟾蜍腓肠肌 等长收缩能力 肌肉损伤 静息张力 松弛速度 

分 类 号：Q4-3[生物学—生理学]