两个水稻胚乳启动子的克隆及表达分析  

Cloning and Expression Analysis of two Endosperm Promoters in Rice

作　　者：崔永兰[1] 钟晓丽[1] 张永明[1] 杨仲南[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《中国生物工程杂志》2008年第8期57-61,共5页China Biotechnology

基　　金：上海高校选拔培养优秀青年教师科研专项基金(RE567);上海市教委基金(06DZ016)资助项目

摘　　要：启动子的克隆对基因表达及基因工程研究有重要意义。根据数据库中EST丰度,从水稻中克隆了两个预测在水稻胚乳中高效表达的启动子Os772和Os359,并将启动子片段与GUS报告基因融合,构建了重组表达载体。通过农杆菌介导方法将其导入水稻愈伤组织细胞。转基因水稻经GUS组织化学分析显示,Os772和Os359能启动GUS基因在水稻胚乳中表达但不能在根、茎、叶和花中表达。该结果表明Os772和Os359为两个水稻胚乳特异性启动子。The cloning of promoter is important for studying the genetic engineering and the regulation of gene expression in plants. Two promoters Os772 and Os359, which are predicted to be highly expressed in the endosperm of rice from the EST database were cloned. After construction of the Os772 ： ： GUS and Os359 ： ： GUS expression vectors, they were transformed into rice. X-Gluc staining of transgenic plants showed that Os772 and Os359 can promote GUS gene expression in matured endosperm but not in root, stem, leaf and flower. This result indicates Os772 and Os359 are two rice endosperm-specific promoters.

关 键 词：水稻 胚乳启动子 GUS报告基因 

分 类 号：Q943.2[生物学—植物学]