超声波萃取烟叶中3-磷酸甘油脱氢酶的研究  

Research in Extraction of GPDH with Ultrasonic from Tobacco Leaf

作　　者：王俊杰[1] 饶志明[1] 沈微[1] 方慧英[1] 诸葛健[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室江南大学生物工程学院工业微生物研究中心,无锡214122

出　　处：《中国生物工程杂志》2008年第8期74-77,共4页China Biotechnology

基　　金：国家自然科学基金(30570142;20676053);国家"863计划"(2006AA020103);江苏省青年科技创新人才基金(BK2006504);长江学者和创新团队发展计划(IRT0532)资助项目

摘　　要：将超声波萃取手段引入到烟叶中3-磷酸甘油脱氢酶提取方法中,并通过正交实验确定了超声波萃取烟叶中3-磷酸甘油脱氢酶(GPDH)的最佳条件为:超声波破碎功率为75w,超声波破碎间歇时间为2s,超声波破碎时间为3s,料液比为1∶15g/ml;在此最佳条件下测得烟叶中3-磷酸甘油脱氢酶为0.3937U/mg蛋白,均比其他提取手段的酶活要高。Nicotiana tabacum is an important and classical model plant which can respond to the change of environmental conditions by accumulating osmoprotectants, such as glycerol and proline which contribute to the re-establishment of homeostasis when exposed to various adverse environmental stresses, such as drought, salinity, high and low temperatures. The optimization of ultrasonic extraction （UE） conditions of glycerol-3- phosphate dehydrogenase （GPDH） of tobacco leaf have been built by orthogonal test. It showed that optimum of the powers, treatment times, slot times and leaf-to-solvent ratios of UE was 75w, 2h, 2s, and 1 ： 12 g/ml, respectively. Under these conditions, the activity of GPDH has been tested as 0. 3937U/mg protein, which was higher than other extraction methods such as liquid nitrogen and grinding on ice bath. According to investigation, it is the first description of determination of content of GPDH with ultrasonic in tobacco. It could provide basis for the further research in the relation of content of glycerol and osmotic pressure in tobacco.

关 键 词：甘油 3-磷酸甘油脱氢酶 超声波 萃取 

分 类 号：Q681[生物学—生物物理学]