金鱼雌核发育单倍体发育过程中的比较蛋白质组学研究  

Comparative Proteome Analysis of Goldfish(Carassius auratus) Gynogenetic Haploid Embryos During Developmental Process

作　　者：张玲[1] 周新文[1] 谢锦云[2] 黄树林[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广州510006 [2]湖南师范大学生命科学学院蛋白质化学实验室,长沙410081

出　　处：《中国生物工程杂志》2008年第8期78-83,共6页China Biotechnology

基　　金：国家自然科学基金(90408017);国家"973"计划(2001CB5120);资助项目

摘　　要：前期已有工作发现在金鱼雌核发育单倍体中一些与发育调控相关的重要蛋白质表达受阻导致单倍体的发育畸形。为了进一步阐明单倍体的发育机制,共收集了3个不同发育时期金鱼单倍体胚胎(HE-1、HE-2、HE-3)进行雌核发育单倍体的差异蛋白质组研究。研究采用二维凝胶电泳进行分离,利用PDQuest软件进行图谱分析,质谱分析初步鉴定到了15个差异蛋白质。这些蛋白质在金鱼雌核发育单倍体的发育中起着重要作用,为进一步阐明单倍体的发育机制奠定了良好的基础。Previous results indicated that the abnormal development of haploid goldfish embryos is due to the expression obstruction of some regulation proteins. Differential display proteomics techniques were employed to study the mechanism of abnormal development in goldfish gynogenetic haploid embryos （HE）. Proteins from three different developmental stages of haploid embryos, called HE -1, HE-2 and HE-3 were separated by two dimensional gel electrophoresis （2-DE） , image analysis was carried out using PDQuest software and 15 proteins were identified by MALDI-TOF/TOF. These proteins play important roles during the development of goldfish gynogenetic haploid embryos. The results might be helpful in further studying the developmental mechanisms of goldfish gynogenetic haploid embryos.

关 键 词：金鱼胚胎 发育过程 双向电泳 蛋白质组 

分 类 号：Q297[生物学—细胞生物学] Q51