净化养殖水体紫色非硫光合细菌的筛选与鉴定  

Screening and Identification of Purple Non-sulfur Photosynthetic Bacteria Purifying Aquaculture Water

作　　者：刘芳[1] 王敏[1] 杨慧[1] 孙军德[2] 高俊莲[1] 

机构地区：[1]北京市农林科学院北京农业生物技术研究中心,北京100097 [2]沈阳农业大学土地与环境学院,沈阳110161

出　　处：《中国生物工程杂志》2008年第8期91-95,共5页China Biotechnology

摘　　要：从杭州养鱼塘水样及底泥样品中富集分离得到3株紫色非硫光合细菌,分别命名为HZ-3,HZ-4,HZ-5。通过比较这3株菌对鱼、虾养殖水体的净化效果,筛选出菌株HZ-5净化能力较强,经过5天的处理,该菌株使养鱼塘水样的COD降低24.87%;使养虾池塘水样的COD降低36.99%;使养鱼塘水样的亚硝态氮的降解率达到97.79%。对菌株HZ-5进行了形态学观察、生理生化鉴定、活细胞吸收光谱以及16S rDNA序列分析。16S rDNA序列分析结果表明该菌株与沼泽红假单胞菌的16SrDNA序列有高达99%的同源性,结合形态特征和生理生化特性以及活细胞吸收光谱特征等,将其鉴定为沼泽红假单胞菌(Rhodopseudomonas palustris)。Three purple non-sulfur bacterial strains were enriched and isolated from the fish pond water and sediments in Hangzhou, they were named HZ-3, HZ-4 and HZ-5 respectively. Among the three purple nonsulfer photosynthetic bacterias, the strain HZ-5 can purify the aquaculture water from fish pond and shrimp pond more efficiently. After 5 days treatment by the strain HZ-5, the COD of fish pond water was decreased by 24.87% ; the COD of shrimp pond water was decreased by 36. 99%; the Nitrite-Nitrogen of fish pond water was decomposed by 97. 79%. The strain HZ-5 was identified as Rhodopseudomonas palustris according to its morphological characteristics, absorption spectrum scanning of live cell and physiological and biochemical properties, and the sequence analysis of 16S rDNA.

关 键 词：养殖水体净化 紫色非硫光合细菌 筛选与鉴定 

分 类 号：Q93-331[生物学—微生物学]