一种新的在线细胞显微观察仪及其在生化反应器上的应用研究  

A Novel In-situ Microscope and Its Application in Bioreactor

作　　者：刘健[1] 孙国强[2] 郭美锦[1] 张岳芳 贺莉清[2] 袁旭军[2] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海理工大学,上海200093 [3]上海科源电子科技有限公司,上海201101

出　　处：《中国生物工程杂志》2008年第8期100-104,共5页China Biotechnology

基　　金：国家"863"计划(2006AA02Z204);上海市生物过程工程平台研究项目(07DZ22914);上海市西部合作项目(074958025);资助项目

摘　　要：针对生化反应器应用条件,提出了用于生化反应器的在线细胞观察仪的基本技术要求。在比较了国内外现有的细胞在线显微工作原理后,研制了一种基于暗视场的新的显微细胞观察仪,介绍了其关键技术及结构。另外,原位在线显微细胞观察仪应用于酿酒酵母和哺乳动物细胞HEK293细胞的培养试验,在线细胞计数结果与离线细胞计数和细胞干重相比较,均有很好的相关性,表明此仪器基本满足使用要求。The technical specifications of in-situ microscope mounted on biochemical reactor were proposed based on the application requirement of biochemical reactor. A novel in-situ microscope was designed and manufactured in dark field principle after all principles of in-situ microscopes used currently had been compared in detail, the designing principle and structure of the in-situ microscope was then described. In addition, it was applied to monitor growth of baker yeast and mammalian cell HEK293, respectively. It indecated the cell amount obtained by in-situ microscope was in a good agreement with dry cell weight and that determined using haemocytometer, suggesting that the new in-situ microscope could satisfy the requirements of high performance used in bioreactor .

关 键 词：在线细胞显微观察仪 生化反应器 细胞形态分析 细胞计数 

分 类 号：Q6-33[生物学—生物物理学]