RP-HPLC法测定大叶香茶菜中冬凌草甲素含量  

Quantitative Determination of Oridonin in Isodon macrophyllus by RP-HPLC

作　　者：王永学[1] 王中全 陈正跃[1] 邱培勇[1] 张积霞[1] 

机构地区：[1]新乡医学院药物研究室,河南新乡453003 [2]河南省新乡市公安局,河南新乡453003

出　　处：《安徽农业科学》2008年第22期9349-9349,9351,共2页Journal of Anhui Agricultural Sciences

基　　金：河南省科技厅科技攻关项目(0624420029)

摘　　要：[目的]应用RP-HPLC法测定大叶香茶菜中冬凌草甲素的含量。[方法]RP-HPLC法色谱条件:色谱柱为Varian Microsorb-MV 100-5C18柱,流动相为甲醇∶水(1∶1),流速1.0 ml/min,检测波长λ=238 nm,柱温30℃。[结果]冬凌草甲素加样回收率为98.6%,RSD为2.04%(n=5);大叶香茶菜叶中冬凌草甲素的含量为4.79 g/kg。[结论]该法简便可靠,适用于大叶香茶菜中冬凌草甲素含量测定。[ Objective] The research aimed to determine the content of ofidonin in Isodon macrophyUus by RP-H1332 method. [Method] The chromatographic conditions of RP-HPLC method were that the chromatographic column was analytical C18 column, the mobile phase was methanol-water（1 ： 1）, the flow rate was 1.0 ml/min, the column temperature was 30 %, the detection wavelength was 238 nm. [Result] The average recovery was 98.6% with RSD 2.04 % （n = 5 ）. The content of ofidonin in Isoclon： macrophyllus was 4.79 g/kg. [ Conclusion ] 3his method was proved to be simple and reliable. It could be used for the oridonin content detemination in Isodon macrophyllus.

关 键 词：大叶香荼菜 冬凌草甲素 RP—HPLC法 

分 类 号：O657.7[理学—分析化学]