临床分离鸡源性大肠杆菌外膜蛋白型的研究  

Studies on Clinical Isolating Outer Membrane Protein Pattern from Avian Escherichia coli

作　　者：张铁[1] 王春光[1] 韩伟[2] 李清艳[1] 吕建存[1] 

机构地区：[1]河北农业大学中兽医学院,河北定州073000 [2]中国农业大学动物医学院,北京100094

出　　处：《安徽农业科学》2008年第22期9520-9522,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省教育厅资助课题(2004322);河北省畜牧兽医局资助课题(2006009)

摘　　要：[目的]了解禽致病性大肠杆菌分离株的遗传相关性。[方法]对分离自保定、秦皇岛和北京3个地区规模化养鸡场的病死鸡体内的38株大肠杆菌,采用超声波裂解和N-十二烷基肌胺酸钠进行外膜蛋白(Omp)提取,利用SDS-PAGE进行Omp分型。[结果]38株大肠杆菌共有3个Omp型,其中Omp-1型为O78、O88、O2、O18、O93、O766个血清型分离株所共有,Omp-Ⅱ型多为O76血清型分离株,Omp-Ⅲ型为O93、O78、O88、O2分离株所共有。这表明同一血清型的菌株可能属于完全不同的Omp型,而血清学上毫不相关的分离株之间却可具有相同的Omp型。[结论]该研究证实了同一血清型的分离株之间可发生遗传分化,而不同血清型的分离株之间也可具有不同程度的遗传相关性。[ Objective] The purpose was to know about the genetic relationship of isolated strains from avian Pathgenicity Escherichia coli （ E. coli ）. [Method] The outer membrane protein （OMP） were extracted from 38 strains of avian E. coli which were isolated from the dead chickens of chicken breeding farms in 3 areas of Baeding, Qiuhuangdao and Beijing by using ultrasonic cleaving and N-Lauroyl Sarcosine Sodium, and OMP typing was done by SDS-PAGE. [Result] There were 3 Omp types in these 38 strains of E. coli, in which, type Omp-1 was in common for six semtype of isolated strains of O78, O88, O2, O18, O93 and O76, type Omp-Ⅱ belonged to isolated strain of 076, Omp-Ⅲ was found from all of the isolated strains of O93, O78, O88, O2. This indicated the identical serotype strain might belong to quite different Omp patterns, and among isolated strains without any relationship in serotype could have the same Omp type. [ Conclusion] Among isolated strains with the same serotype strains might have genetic differentiation. And among the isolated strains of different serotypes, there might be the genetic relationship with different degree.

关 键 词：鸡大肠杆菌 外膜蛋白 O血清型 

分 类 号：S858.31[农业科学—临床兽医学]