北冬虫夏草液体深层发酵最适营养的研究  

Tissue Culture and Clonal Propagation of Nine Plants in Vitro

作　　者：李维光[1,2,3] 娄虹[1,2,3] 林建群[1,2,3] 郝淑凤 苏凤岩[1,2,3] 

机构地区：[1]中国科学院沈阳应用生态研究所 [2]辽宁大学生物系 [3]山东青岛国箭公司

出　　处：《辽宁大学学报（自然科学版）》1997年第3期34-38,共5页Journal of Liaoning University：Natural Sciences Edition

摘　　要：通过摇瓶单因子和正交设计试验结果，液体培养的最佳营养条件为：葡萄糖２％，食用糖１％，酵母膏１％，蛋白胨１％，ＮＨ４Ｃｌ０．２％，ＭｇＳＯ４·７Ｈ２Ｏ０．０５％，ＫＣｌ０．１％；１００立升发酵罐结果，碳、氮源分别为食用糖３％，蛋白胨１．５％．We take the petal of cymbidium, stem tip of chrysanthemum, Rosachintnsis, Leaflet of platycodon grandiflorum, primula obconica, petunia hybrida, root of panax ginsting, anther of oryzasatina, and embryo of Pimus Roraicnsis as material, and then inoculate them respectively with culture medium (See the Table 1,2). The explants for the first time and through the successive and differentiation culture, the protocorm from cymbidium were regenerated plantlets; the chrysanthemum and Rosachintnsis induced into thickly buds. Finally they differentiated into plantlet; the platycodon grandifcorum, primula obconica and petunia hybrida were induced into calli. They differentiated adventitous bud (ro embryoid), and then formed regenerated plantlets. The oryzasatiza were induced into calli. They differentiated into embryoids. Finally they formed regenerated plantlets.

关 键 词：北冬睡草 液体深层发酵 菌丝体 营养成分 

分 类 号：TQ929[轻工技术与工程—发酵工程]