HPLC法测定秦艽炮制品中龙胆苦苷的含量  

Determination of Gentiopicroside in Processed Radix Gentianae Macrophyllae by HPLC

作　　者：何禄仁[1] 赵建邦[1] 马潇[1] 朱俊儒[1] 张红成[2] 

机构地区：[1]甘肃省药品检验所,兰州730000 [2]兰州大学药学院

出　　处：《中国药事》2008年第8期689-691,共3页Chinese Pharmaceutical Affairs

摘　　要：通过测定秦艽及秦艽炮制品中龙胆苦苷的含量,以探讨炮制方法对秦艽中龙胆苦苷的含量影响。采用高效液相色谱法,Waters C18色谱柱;流动相：甲醇-水（3∶7）;流速：1.0mL·min-1;检测波长：272nm。龙胆苦苷在1.104~8.280μg范围内具有良好线性关系,平均回收率为102.23%（RSD=2.9%）。不同的炮制方法对秦艽中龙胆苦苷含量有一定影响。It investigated the effects of processing to determine the content of gentiopieroside in processed Radix gentianae macrophyllae. The HPLC methods used Waters C18 column, methanol-water （3 ： 7） as the mobile phase. The flow rate was 1.0mL · min-1 at the deteclion wavelength of 272nm. For gentiopicroside, the linear range was 1. 104-8. 280μg and the average recovery was 102.23 % with RSD=2.9%. The processing had effects on the contents of gentiopicroside in Radix gentianae macrophgllae.

关 键 词：秦艽 炮制 龙胆苦苷 HPLC 

分 类 号：R927.2[医药卫生—药学] R284.1