液相色谱-质谱联用测定大鼠血浆中芍药内酯苷及其药动学研究  

Determination of Albiflorin in Rat Plasma by LC-MS/MS and Its Pharmacokinetics

作　　者：王巧[1] 杨汉煜 刘伟娜[1] 李小娜[1] 刘荣霞[3] 毕开顺[4] 果德安[3] 

机构地区：[1]河北医科大学药学院,石家庄050017 [2]石家庄中奇制药技术有限公司,石家庄050000 [3]北京大学药学院,北京100083 [4]沈阳药科大学药学院,沈阳110016

出　　处：《中国药学杂志》2008年第15期1170-1173,共4页Chinese Pharmaceutical Journal

基　　金：河北省自然科学基金基地专项(08B031);中医药管理局资助项目(2004ZX01);河北省教育厅项目(2006133)

摘　　要：目的建立大鼠血浆中芍药内酯苷的HPLC-MS/MS定量分析方法,并用于大鼠体内芍药内酯苷药动学研究。方法血浆样品采用固相萃取预处理。色谱柱为Symmetry C18(4.6mm×50mm,3.5μm),流动相为乙腈-0.05%甲酸溶液(25∶75)。采用三重四极杆质谱仪进行多反应监测方式(MRM),电喷雾离子源。对所建方法进行了较全面的验证。考察了3种剂量芍药内酯苷给与大鼠后的药动学特征。结果所建方法灵敏、重现性良好。芍药内酯苷在体内吸收迅速,消除也较快。经统计学检验表明,在给定的3个剂量范围内,芍药内酯苷具有线性动力学特征。结论建立的方法可用于芍药内酯苷的药动学研究。OBJECTIVE To develop a rapid, sensitive and selective liquid chromatography tandem spectrometry method for the determination of albiflorin in rat plasma, and to investigate its pharmacokinetic parameters in rats. METHODS The plasma samples were pretreated with solid phase extraction. HPLC analysis was carried out on a Symmetry Cts column （4. 6 mm x50 mm, 3.5 μm） with a mobile phase consisting of acetonitrile and 0. 05% formic acid （ 25 ： 75 ） . Detection was carried out on a triple quadrupole tandem mass spectrometer by multiple-reaction monitoring and an electrospray ionization source was employed as the ionization source. The method was fully validated. The phamacokinetic characteristics of albiflorin was investigated after oral administration to rats in three dosages. RESULTS The method was sensitive and reproducable. Albiflorin was absorbed and eliminated rapidly. The statistic analysis showed the pharmacokinetics of albiflorin complied with linear kinetic course. CONCLUSION The method could be applied to the study of pharmacokinetics of albiflorin.

关 键 词：液相色谱-质谱联用 芍药内酯苷 药动学 

分 类 号：R917[医药卫生—药物分析学]