HPLC法同时测定舒胆胶囊中4种黄酮类成分的含量  

HPLC simultaneous determination of four flavonoid in Shudan capsules

作　　者：陈丽姑[1] 杨丽 王冬梅[1] 徐海燕[1] 刘晓英[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]辽宁电力中心医院药剂科,沈阳110015

出　　处：《药物分析杂志》2008年第8期1234-1237,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立 HPLC 法同时测定舒胆胶囊中柚皮苷、橙皮苷、新橙皮苷和黄芩苷4种黄酮类成分的含量。方法:采用 Dia—monsil C_(18)柱(5μm,200 mm×4.6 mm)柱分离,以甲醇-0.2%磷酸水溶液进行梯度洗脱,洗脱程序为:0～35 min,甲醇34%;35～44 min,甲醇38%;44～60 min,甲醇48%。在283 nm下进行测定。结果:柚皮苷、橙皮苷、新橙皮苷和黄芩苷的线性范围分别为9.0～180.0μg·mL^(-1)(r=0.9999,n=5).0.8～16.0μg·mL^(-1)(r=0.9998,n=5),15.4～308.0μg·mL^(-1)(r=0.9998,n=5),20.0～400.0μg·mL^(-1)(r=0.9999,n=5);平均回收率分别为100.0%,99.2%,100.5%,101.2%(n=9)。结论:本方法简便、快捷,结果准确、可靠,重复性好,可作为舒胆胶囊的质量控制方法之一。Objective ： To establish an HPLC method for simuhaneous detennnination of naringin,hesperidin,neohesperidin and baiealin in Shudan capsules. Methods：The chromatographic method was carried out on a Diamonsil C18 column （5 μm,200 mm × 4. 6 mm） with methanol -0. 2% phosphoric acid solution as the mobile phase by gradient elution. The gradient program of solvent was as follows ：0 -35 min,methanol 34% ;35 -44 min,methanol 38% ; 44 -60 min, methanol 48%. The detection was set at the wavelength of 283 nm. Results：The linear ranges of naringin, hesperidin, neohesperidin and baiealin were 9.0 - 180. 0 μg·mL^-1 （ r = 0. 9999, n = 5 ） , 0. 8 - 16.0μg·mL^-1 （r =0. 9998,n =5） ,15.4 -308.0 μg·mL^-1 （r =0. 9998,n =5） ,20. 0 -400. 0 μg·mL^-1 （r =0. 9999,n =5） ,respectively. The average recoveries were 100.0% ,99.2% , 100.5% , 101.2% （ n = 9 ） , respectively. Conclusions ： The method is simple, accurate and reproducible, which can be used for the quality control of Shudan capsules.

关 键 词：HPLC 舒胆胶囊 柚皮苷 橙皮苷 新橙皮苷 黄芩苷 

分 类 号：R917[医药卫生—药物分析学]