HPLC法研究奥卡西平及代谢产物在小鼠体内的组织分布  

HPLC study on tissue distribution of oxcarbazepine and its active metabolite in mouse

作　　者：周红宇[1] 黄成坷[1] 游一玉[1] 胡爱萍[1] 胡国新[1] 

机构地区：[1]温州医学院药学院药理教研室,温州325027

出　　处：《药物分析杂志》2008年第8期1256-1260,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：温州市科技局项目(WKW03073)

摘　　要：目的:建立小鼠血浆和组织中奥卡西平(oxcarbazepine,OXC)及其活性代谢产物10-羟基卡马西平(10-hydroxycarbaz—epine,MHD)的 HPLC 分析方法,并研究其小鼠体内的组织分布。方法:选择卡马西平为内标,采用 Hypersil—ODS C_(18)(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-水(63:37)为流动相,流速0.8 mL·min^(-1),柱温30℃,在紫外波长为210 nm 处检测。所得数据用 DAS 药动学程序处理,求出有关的药动学参数。结果:血浆 OXC 和 MHD 在0.05～10.0 mg·L^(-1)的浓度范围内线性关系良好(r=0.9994),相对回收率为96.4%～107.6%,绝对回收率为75.1%～88.3%,日内、日间精密度的 RSD 均小于10%。OXC 和 MHD 在脂肪、心脏、脑及肝脏等组织分布较多,在血浆和多数组织中 OXC 的 C_(max)高,T_(max)、AUC 和 MRT 较小;而 MHD的 C_(max)低,T_(max)、AUC 和 MRT 明显大于 OXC。结论:该方法简便、准确、灵敏度高,可用于 OXC 和 MHD 的检测及药动学研究。Objective： To establish an HPLC method for analysis of tissue distribution of oxcarbazepine （ OXC ） and its active metabolite（ 10 -hydroxycarbazepine, MHD） in mouse. Method：With carbamazepine as the internal standard,OXC and MHD were detectded by UV detector at 210 nm with the Hypersil- ODS C18 column and acetonitrile -water（63： 37） as the mobile phase at a flow rate of 0.8 mL·min5-1. The data obtained with DAS program on computer. Results：The linear range, of OXC and MHD in plasma were 0.05 - 10.0 mg · L^-1 （ r = 0. 9994）. The relative recoveries of the assay were 96.4% - 107.6%. The absolute recoveries of the assay were 75.1% - 88.3%. Both the intra - day and inter - day validations were less than 10%. The AUC values of OXC and MHD in the fat, heart, brain, lung and liver were more than that in other tissues. In the plasma, lung, brain, muscle and fat, the Cmax of OXC was larger than that of MHD, and the Tmax, AUC, MRT of OXC was little than that of MHD. Conclusion ： This method is simple, sensitive and accurate. It can be used for the investigation of OXC and MHD in mouse.

关 键 词：高效液相色谱法 奥卡西平 10-羟基卡马西平 小鼠 组织分布 

分 类 号：R917[医药卫生—药物分析学]