高效毛细管电泳测定大鼠血浆中青蒿素的含量  

Determination of artemisinin in rat plasma by capillary electrophoresis with amperometric detection

作　　者：黄宝美[1] 姚程炜 莫金垣[3] 

机构地区：[1]绵阳师范学院化学系,绵阳621000 [2]中国气动中心第四研究所,绵阳622653 [3]中山大学化学与化学工程学院,广州510275

出　　处：《药物分析杂志》2008年第8期1330-1332,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立了血浆中青蒿素的高效毛细管电泳检测方法。方法:采用 Tris—H_3BO_3(H_3BO_3浓度为1.5 mmol·L^(-1))为电泳介质,乙醇为有机添加剂,在15kV 高压,pH 9.0的碱性条件下柱端伏安检测了青蒿中的青蒿素含量。探讨了缓冲溶液种类、浓度、酸碱度,有机添加剂及其操作电压、进样时间等对检测的影响。结果:该法的线性范围为:150～1.5 mg·L^(-1),r=0.9995,检出浓度为0.5 mg·L^(-1)。结论:该法简便、快速、准确,可用于人体血浆中青蒿素的浓度测定。Objective： A method for determination of artemisinin in human plasma was established by high performance capillary electrophoresis using self- designed amperometric detection system. Methods：Several parameters affecting the determination were studied, including effect of running buffer concentration, acidity, sampling time and separation voltage. Results： Under the following conditions ： Tris - H3 BO3 （ H3 BO3 1.5 mmol·L^ - 1, pH = 8. 5 ）, 15 kV running voltage,a linearity exists between peak height and mass concentration in the range of 1.5 - 150 mg · L^- 1, determination limit was 0. 5 mg · L^-1. Conclusions：This method is simple, rapid and accurate.

关 键 词：毛细管电泳 青蒿素 伏安检测 血浆 

分 类 号：R917[医药卫生—药物分析学]