心肌匀浆上清液对大鼠骨骼肌卫星细胞诱导分化的影响  

Effect of myocardial homogenate on transdifferentiation of muscle satellite cells of rats

作　　者：徐文忠[1] 杨苏民[1] 刘旭[1] 吴玉辉[1] 

机构地区：[1]青岛大学医学院附属医院,266101

出　　处：《实用医学杂志》2008年第17期2957-2959,共3页The Journal of Practical Medicine

摘　　要：目的:探讨心肌匀浆上清液对大鼠骨骼肌卫星细胞诱导分化的影响。方法:体外培养的大鼠骨骼肌卫星细胞,检测纯度。于原代培养的第3天加入心肌匀浆液持续作用1周,3周后观察细胞形态。采用免疫组织化学的方法检测心肌特异性肌钙蛋白-T(cardiac troponin-T,cTn-T)的表达。结果:诱导培养后的大鼠骨骼肌卫星细胞不表达cTn-T蛋白。结论:骨骼肌卫星细胞在心肌匀浆诱导下没有向心肌细胞转化。Objective To investigate the effects of myocardial homogenate of rats on transdifferentiation of muscle satellite cells（SCs）. Methods After establishing stable culture methods for rats SCs and identification of SCs, they were induced by myocardial homogenate on day 3 of primary culture for 1 week. Imumunocytochemistry technique was used to detect troponin-T（cTn-T） 3 weeks later. Result SCs of rats did not express cardiac cTn-T after induction. Conclusion Myocardial homogenate cannot induce SCs of rats to transdifferentiate into cardiomyocytes.

关 键 词：肌 骨骼 卫星细胞 细胞分化 大鼠 心肌匀浆 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]